ProtParam
User-provided sequence:
10 20 30 40 50 60
MDFTAKIKEL ERELSETSDY KTLQKKTISL RSELNTLSHS LTSYEKEHFS NDIENVLKSI
70 80 90 100 110 120
NAKLSESKGK KRLFSFKQKN SSSAVHKNVE RTELANAPAY TTTLKKHYVL EKGDSAFENL
130 140 150 160 170 180
EFCTVTSTTD YSGNSALSGS LCFRNITKCV INLQRIFFQT GSIFITDCTD SIIFLRSPSD
190 200 210 220 230 240
KDFQIRLRDL KNCKILIEKL SPSIDCKQVV IIENCHKCIF NASTRDHLII QDFSNPFQSE
250 260
ETEDNSAFAF EDFDICNKDT MQLFRAYL
References and
documentation are available.
Number of amino acids: 268
Molecular weight: 30708.78
Theoretical pI: 6.61
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1354
Hydrogen H 2146
Nitrogen N 364
Oxygen O 427
Sulfur S 11
Formula: C
1354H
2146N
364O
427S
11
Total number of atoms: 4302
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9440
Abs 0.1% (=1 g/l) 0.307, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.291, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.99
This classifies the protein as unstable.
Aliphatic index: 80.07
Grand average of hydropathicity (GRAVY): -0.441