ProtParam
User-provided sequence:
10 20 30 40 50 60
MDFWPEFMAT SWGREFVAGG FGGVAGIISG YPLDTLRIRQ QQSSKSGSAF SILRRMLAIE
70 80 90 100 110 120
GPSSLYRGMA APLASVTFQN AMVFQIYAIF SRSFDSSVPL VEPPSYRGVA LGGVATGAVQ
130 140 150 160 170 180
SLLLTPVELI KIRLQLQQTK SGPITLAKSI LRRQGLQGLY RGLTITVLRD APAHGLYFWT
190 200 210 220 230 240
YEYVRERLHP GCRKTGQENL RTMLVAGGLA GVASWVACYP LDVVKTRLQQ GHGAYEGIAD
250 260 270 280 290
CFRKSVKQEG YTVLWRGLGT AVARAFVVNG AIFAAYEVAL RCLFNQSPSP DIVTGD
References and
documentation are available.
Number of amino acids: 296
Molecular weight: 32252.30
Theoretical pI: 9.69
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1459
Hydrogen H 2293
Nitrogen N 401
Oxygen O 405
Sulfur S 10
Formula: C
1459H
2293N
401O
405S
10
Total number of atoms: 4568
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45630
Abs 0.1% (=1 g/l) 1.415, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.407, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.10
This classifies the protein as unstable.
Aliphatic index: 93.92
Grand average of hydropathicity (GRAVY): 0.130