ProtParam
User-provided sequence:
10 20 30 40 50 60
MDGAKFENTV AFLPSEIFDC YNSTLPKNVF RSFVTWSCYE KFNSLEFRTW LLMWLPLIIA
70 80 90 100 110 120
WKIRGKRHYL VIVTALMFEV LYFLWTYSYI FRERTLGKQV SQFAKEIITN TPGIDTEDWE
130 140 150 160 170 180
RVAVNFNSYL YENKLWNTEY FFFDGSSCQE AFRKMLLEPF SLKKNDFANA KVPDGSVCYT
190 200 210 220 230 240
EKALQVYFTQ IERKWHWINS EGFLHNKTTQ SVQFSKHGYG SKLLWAFKEV TIMNSRFAFF
250 260 270 280 290 300
SIAYLNGLLT IPRLRNSLHI LYVCAVLSSM IIEYLIGIDK FRFKSMNLIH KLQFLSYITC
310 320 330 340 350 360
GHEKSDATNW SQIAKRTNTY MFEQKIWNSP ILFSDGIDCE KFFKWYFSTP VSSQASLSVG
370 380
STDFELWPYI KEAQSACNDV
References and
documentation are available.
Number of amino acids: 380
Molecular weight: 44803.58
Theoretical pI: 8.60
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 2084
Hydrogen H 3100
Nitrogen N 510
Oxygen O 562
Sulfur S 16
Formula: C
2084H
3100N
510O
562S
16
Total number of atoms: 6272
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 105810
Abs 0.1% (=1 g/l) 2.362, assuming all pairs of Cys residues form cystines
Ext. coefficient 105310
Abs 0.1% (=1 g/l) 2.350, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.00
This classifies the protein as stable.
Aliphatic index: 82.11
Grand average of hydropathicity (GRAVY): -0.105