ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGEMAIIES SNHVGTSSPT TETQFTIDSS ALKDQETKES ITNSPTSEVP IETKLPKSSD 

        70         80         90        100        110        120 
IVTEEKHPQN TTTDIENEVE NPVTDDNGNL KLELPDNLDN ADFSKLLEFD AKNDEALFNS 

       130        140        150        160        170        180 
NELLSHTMDP VNNIDLTHDH SREVSSKEDI NIEPVNPDED EREKTQDNTA AVKTEGIRNS 

       190        200        210        220        230        240 
EDTSIQKDEP TADAIYTDVH KLSVNKDTET LPTLVDEKNN MLHMRNNSIT PIMFQQHELV 

       250        260        270        280        290        300 
GQPPQNTVTE NNSTDAETTQ RKLSEPIDAS LPLPNEQPTI FAYARLDFQS FTFYVQTLHA 

       310        320        330        340        350        360 
IIGRRSENDF SHKVDVNLGP SKSISRRHAQ IFYNFGTGRF ELSIIGKNGA FVDDIFVEKG 

       370        380        390        400        410        420 
NTVPLRNKTK IQIGQIPFQF ILPEQERNDD SKSPENADIA ESEINTRNLK KNEPKSKKKI 

       430        440        450        460        470        480 
TTGAKPKKAQ TKPAVKKEKK PPKIPKKVYT LEEIPVEYRT KPTVSYSAML TTCIRKYSTA 

       490        500        510        520        530        540 
KGMSLSEIYA GIRELFPYYK YCPDGWQSSV RHNLSLNKSF RKVSKEGKGW LWGLDEEYIA 

       550        560        570        580        590        600 
ERERQKKKQS EIAVAKAQAA QLKLEQQQHK LQQVPQRGKK DIVSQRSNVN ARKQNISQTL 

       610        620        630        640        650        660 
AANRAASNRK NTASDNQRTM KYLQEQLVIL TRDRKGLSKQ VIAAILTQAL AMTINQVTQA 

       670        680        690        700        710        720 
AKNKGITGNP LTALMDKNPQ HLNLILAAAV NAATAKVTKG EVKQLVNPET TAAAALAAKA 

       730        740        750        760        770        780 
QHSKPIRQPI VQTPHVPDRP PSQLSASASS HPNNYLHDKQ PGSFDPSSLS RFFQPRQNAR 

       790        800        810        820        830        840 
ATSSVAATSV PAAASQNVDA QPKPKPAQDN DLESESGTSS SSSSSSESGS ESDSGSDDGS 

       850        860        870        880        890        900 
ASGSGDNSST SSESESESDS GSEVDEKNNK NEKIDSESIK NNESKDDIPS KDENSSNDNR 

       910        920        930 
EISKTDEEGH DSKRRKVSED INEGITEVNV SLEEKL 

References and documentation are available.

Number of amino acids: 936

Molecular weight: 103502.36

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  68	  7.3%
Arg (R)  37	  4.0%
Asn (N)  70	  7.5%
Asp (D)  61	  6.5%
Cys (C)   2	  0.2%
Gln (Q)  52	  5.6%
Glu (E)  78	  8.3%
Gly (G)  35	  3.7%
His (H)  19	  2.0%
Ile (I)  54	  5.8%
Leu (L)  58	  6.2%
Lys (K)  82	  8.8%
Met (M)  11	  1.2%
Phe (F)  22	  2.4%
Pro (P)  53	  5.7%
Ser (S) 103	 11.0%
Thr (T)  67	  7.2%
Trp (W)   3	  0.3%
Tyr (Y)  15	  1.6%
Val (V)  46	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 139
Total number of positively charged residues (Arg + Lys): 119

Atomic composition:

Carbon      C	      4447
Hydrogen    H	      7168
Nitrogen    N	      1292
Oxygen      O	      1522
Sulfur      S	        13

Formula: C4447H7168N1292O1522S13
Total number of atoms: 14442

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38975
Abs 0.1% (=1 g/l)   0.377, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.375, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.80
This classifies the protein as unstable.



Aliphatic index: 68.18

Grand average of hydropathicity (GRAVY): -0.902