ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGLKNIKKV LVANRGEIAI RIFRACTELH IRTVAIYSKE DTGAYHRYKA DEAYLVGEGK 

        70         80         90        100        110        120 
KPIEAYLDIE GIIEIAKRHG VDAIHPGYGF LSENIEFAKR CHEEGIIFIG PELEHLVMFG 

       130        140        150        160        170        180 
DKVQAREQAI KANLPVIPGS DGPVSSLEDV KAFADKHGYP FIIKAALGGG GRGMRIVRSE 

       190        200        210        220        230        240 
NDVQESYERA KSEAKAAFGN DEVYVEKFIE NPKHIEVQIL ADKHGNTLHL YERDCSVQRR 

       250        260        270        280        290        300 
HQKVVEVAPS VSLSEDVRER ICQAAVQLAE NVNYVNAGTV EFLVDREGNF YFIEVNPRIQ 

       310        320        330        340        350        360 
VEHTITEMVT GIDIVQSQLF IADGEHLHGD RLGIPKQEEI VCHGYAIQSR VTTEDPSNGF 

       370        380        390        400        410        420 
LPDTGRINAY RSGGGFGVRL DAGNGFQGAV ITPYYDSLLV KVSTWALTFE GAAAKMLRNL 

       430        440        450        460        470        480 
REFRIRGIKT NIAFLENVVQ HRQFLSGEYN TSFIDQTPEL FVFPKRKDRG TKMLSFIGET 

       490        500        510        520        530        540 
IVNGYPGLEK TKKPVFDKPP VPKLKLSEPI PDGTKQILDQ HGPEGLAKWV KEQKHVLLTD 

       550        560        570        580        590        600 
TTFRDAHQSL LATRVRTHDL KQIAEPTARL LPNLFSMEMW GGATFDVAMR FLHEDPWERL 

       610        620        630        640        650        660 
LILRKKAPNV LFQMLLRASN AVGYKNYPDN LIREFVDKSA NAGIDVFRIF DSLNWVEGMK 

       670        680        690        700        710        720 
LAIEAVGEAN KIAEATICYT GDILDSSRPK YDLAYYKKLA KELEAAGAHI LGIKDMAGLL 

       730        740        750        760        770        780 
KPEAAYQLVA ELKDTVTIPV HLHTHDTSGN GIFTYARAIE AGVDIVDVAV SSMAGLTSQP 

       790        800        810        820        830        840 
SANSLYYALA DSERQPNVNI TALEQLAEFW EETRKFYAGF ESGMNAPHTE VYEHEMPGGQ 

       850        860        870        880        890        900 
YSNLQQQAKA VGLGHRWNEV KKMYRTVNDM FGDVVKVTPS SKVVGDMALY MVQNDLTEEE 

       910        920        930        940        950        960 
VYENGHKLDF PDSVVEFFEG QLGQPYQGFP KKLQEIILKG RKPITNRPGE NMEPIQFEAI 

       970        980        990       1000       1010       1020 
KEELYNKLDR QVTSHDILSY ALYPKVFMEF ERFRQTFGDV SVLDTPTFFY GLRPGEEIEV 

      1030       1040       1050       1060       1070       1080 
EIEQGKTLIV KFISLSKPQD DGNRIVYFEL NGQPREVLIK DQSVKTSIIS RPKADKSNPN 

      1090       1100       1110       1120       1130       1140 
HIGASMPGTV VKALVEKGDK VKQGDHLMIT EAMKMETTVQ APFDGEVVAL HVKDGDAIQT 

      1150 
GDLLIEVKEL 

References and documentation are available.

Number of amino acids: 1150

Molecular weight: 128858.88

Theoretical pI: 5.68

Amino acid composition: 
Ala (A)  88	  7.7%
Arg (R)  56	  4.9%
Asn (N)  46	  4.0%
Asp (D)  65	  5.7%
Cys (C)   6	  0.5%
Gln (Q)  46	  4.0%
Glu (E)  99	  8.6%
Gly (G)  89	  7.7%
His (H)  33	  2.9%
Ile (I)  78	  6.8%
Leu (L)  93	  8.1%
Lys (K)  79	  6.9%
Met (M)  25	  2.2%
Phe (F)  52	  4.5%
Pro (P)  52	  4.5%
Ser (S)  51	  4.4%
Thr (T)  54	  4.7%
Trp (W)   7	  0.6%
Tyr (Y)  40	  3.5%
Val (V)  91	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 164
Total number of positively charged residues (Arg + Lys): 135

Atomic composition:

Carbon      C	      5777
Hydrogen    H	      9072
Nitrogen    N	      1562
Oxygen      O	      1716
Sulfur      S	        31

Formula: C5777H9072N1562O1716S31
Total number of atoms: 18158

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    98475
Abs 0.1% (=1 g/l)   0.764, assuming all pairs of Cys residues form cystines


Ext. coefficient    98100
Abs 0.1% (=1 g/l)   0.761, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.89
This classifies the protein as stable.



Aliphatic index: 88.59

Grand average of hydropathicity (GRAVY): -0.317