ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGLNWLLHS WCKENSVILA DEMGLGKTIQ TICFLYSLFK IHHLYGPFLC VVPLSTMTAW 

        70         80         90        100        110        120 
QREFDLWAPD MNVVTYLGDI KSRELIQQYE WQFESSKRLK FNCILTTYEI VLKDKQFLGT 

       130        140        150        160        170        180 
LQWAALLVDE AHRLKNDDSL LYKSLKEFDT NHRLLITGTP LQNSLKELWA LLHFIMPDKF 

       190        200        210        220        230        240 
DTWENFEVQH GNAEDKGYTR LHQQLEPYIL RRVKKDVEKS LPAKVEQILR VEMTSLQKQY 

       250        260        270        280        290        300 
YKWILTKNFD ALRKGKRGST STFLNIVIEL KKCCNHAALI RPSEFELMGL QQDEALQTLL 

       310        320        330        340        350        360 
KGSGKLVLLD KLLCRLKETG HRVLIFSQMV RMLDVLADYL QKRHFPFQRL DGSIKGEMRR 

       370        380        390        400        410        420 
QALDHFNAEG SQDFCFLLST RAGGLGINLA TADTVIIFDS DWNPQNDLQA QARAHRIGQK 

       430        440        450        460        470        480 
NQVNIYRLVT ARSVEEQIVE RAKQKMVLDH LVIQRMDTTG RTVLDKSGNG HSSNSNPFNK 

       490        500        510        520        530        540 
DDLSAILKFG AEELFKDEQE HDDDLVCDID EILRRAETRN EDPEMPADDL LSAFKVASIA 

       550        560        570        580        590        600 
AFEEEPSDSV SKQDQNAAGE EDDSKDWDDI IPEGFRKAID DQERAKEMED LYLPPRRKTA 

       610        620        630        640        650        660 
ANQNEGKRGA GKGGKGKQQA DDSGGDSDYE LGSDGSGDDG RPRKRGRPTM KEKITGFTDA 

       670        680        690        700        710        720 
ELRRFIRSYK KFPAPLHRME AIACDAELQE KPLAELKRLG EMLHDRCVQF LHEHKEEESK 

       730        740        750        760        770        780 
TAATDETPGA KQRRARATFS VKLGGVSFNA KKLLACEQEL QPLNEIMPSM PEERQQWSFN 

       790        800        810        820        830        840 
IKTRAPVFDV DWGIEEDTKL LCGIYQYGIG SWEQMKLDPT LKLTDKILLN DTRKPQAKQL 

       850        860        870        880        890        900 
QTRAEYLLKI IKKNVELTKG GQRRQRRPRA SRANDAKAAS QSASSTIDAK PHDGEDAAGD 

       910        920        930        940        950        960 
ARTVAESSNS QVDPSTASPH NAPATEQHGD PAKKAKKSKA RSKKTSASDN NGNKPMHFTA 

       970        980        990       1000       1010       1020 
NNEPRALEVL GDLDPSIFNE CKEKMRPVKK ALKALDQPDV SLSDQDQLQH TRDCLLQIGK 

      1030       1040       1050       1060       1070       1080 
QIDVCLNPYA ETEKKEWRSN LWYFVSKFTE LDAKRLFKIY KHALKQKAGG DGEAKGKDKG 

      1090       1100 
SSGSPAKSKP NGVTTEKKKK K 

References and documentation are available.

Number of amino acids: 1101

Molecular weight: 124999.90

Theoretical pI: 8.09

Amino acid composition: 
Ala (A)  84	  7.6%
Arg (R)  65	  5.9%
Asn (N)  43	  3.9%
Asp (D)  86	  7.8%
Cys (C)  16	  1.5%
Gln (Q)  61	  5.5%
Glu (E)  81	  7.4%
Gly (G)  65	  5.9%
His (H)  26	  2.4%
Ile (I)  48	  4.4%
Leu (L) 116	 10.5%
Lys (K) 106	  9.6%
Met (M)  22	  2.0%
Phe (F)  40	  3.6%
Pro (P)  43	  3.9%
Ser (S)  67	  6.1%
Thr (T)  53	  4.8%
Trp (W)  16	  1.5%
Tyr (Y)  21	  1.9%
Val (V)  42	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 167
Total number of positively charged residues (Arg + Lys): 171

Atomic composition:

Carbon      C	      5495
Hydrogen    H	      8770
Nitrogen    N	      1574
Oxygen      O	      1681
Sulfur      S	        38

Formula: C5495H8770N1574O1681S38
Total number of atoms: 17558

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   120290
Abs 0.1% (=1 g/l)   0.962, assuming all pairs of Cys residues form cystines


Ext. coefficient   119290
Abs 0.1% (=1 g/l)   0.954, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.50
This classifies the protein as unstable.



Aliphatic index: 76.78

Grand average of hydropathicity (GRAVY): -0.714