ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGMYNFHSA GDYSDKSVLM MSPESLMFPS DYQALLCSSA GENRVSDVFG SDELLSVAVS 

        70         80         90        100        110        120 
ALSSEAASIA PEIRRNDDNV SLTVIKAKIA CHPSYPRLLQ AYIDCQKVGA PPEIACLLEE 

       130        140        150        160        170        180 
IQRESDVYKQ EVVPSSCFGA DPELDEFMET YCDILVKYKS DLARPFDEAT CFLNKIEMQL 

       190        200        210        220        230        240 
RNLCTGVESA RGVSEDGVIS SDEELSGGDH EVAEDGRQRC EDRDLKDRLL RKFGSRISTL 

       250        260        270        280        290        300 
KLEFSKKKKK GKLPREARQA LLDWWNLHYK WPYPTEGDKI ALADATGLDQ KQINNWFINQ 

       310        320 
RKRHWKPSEN MPFAMMDDSS GSFFTEE 

References and documentation are available.

Number of amino acids: 327

Molecular weight: 36933.60

Theoretical pI: 4.87

Amino acid composition: 
Ala (A)  24	  7.3%
Arg (R)  18	  5.5%
Asn (N)  11	  3.4%
Asp (D)  28	  8.6%
Cys (C)   9	  2.8%
Gln (Q)  11	  3.4%
Glu (E)  28	  8.6%
Gly (G)  17	  5.2%
His (H)   5	  1.5%
Ile (I)  14	  4.3%
Leu (L)  30	  9.2%
Lys (K)  22	  6.7%
Met (M)  10	  3.1%
Phe (F)  13	  4.0%
Pro (P)  15	  4.6%
Ser (S)  33	 10.1%
Thr (T)   8	  2.4%
Trp (W)   5	  1.5%
Tyr (Y)  10	  3.1%
Val (V)  16	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1616
Hydrogen    H	      2524
Nitrogen    N	       440
Oxygen      O	       513
Sulfur      S	        19

Formula: C1616H2524N440O513S19
Total number of atoms: 5112

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42900
Abs 0.1% (=1 g/l)   1.162, assuming all pairs of Cys residues form cystines


Ext. coefficient    42400
Abs 0.1% (=1 g/l)   1.148, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.12
This classifies the protein as unstable.



Aliphatic index: 74.01

Grand average of hydropathicity (GRAVY): -0.522