ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGPNFAHQG GRSQRTTELY SCARCRKLKK KCGKQIPTCA NCDKNGAHCS YPGRAPRRTK 

        70         80         90        100        110        120 
KELADAMLRG EYVPVKRNKK VGKSPLSTKS MPNSSSPLSA NGAITPGFSP YENDDAHKMK 

       130        140        150        160        170        180 
QLKPSDPINL VMGASPNSSE GVSSLISVLT SLNDNSNPSS HLSSNENSMI PSRSLPASVQ 

       190        200        210        220        230        240 
QSSTTSSFGG YNTPSPLISS HVPANAQAVP LQNNNRNTSN GDNGSNVNHD NNNGSTNTPQ 

       250        260        270        280        290        300 
LSLTPYANNS APNGKFDSVP VDASSIEFET MSCCFKGGRT TSWVREDGSF KSIDRSLLDR 

       310        320        330        340        350        360 
FIAAYFKHNH RLFPMIDKIA FLNDAATITD FERLYDNKNY PDSFVFKVYM IMAIGCTTLQ 

       370        380        390        400        410        420 
RAGMVSQDEE CLSEHLAFLA MKKFRSVIIL QDIETVRCLL LLGIYSFFEP KGSSSWTISG 

       430        440        450        460        470        480 
IIMRLTIGLG LNRELTAKKL KSMSALEAEA RYRVFWSAYC FERLVCTSLG RISGIDDEDI 

       490        500        510        520        530        540 
TVPLPRALYV DERDDLEMTK LMISLRKMGG RIYKQVHSVS AGRQKLTIEQ KQEIISGLRK 

       550        560        570        580        590        600 
ELDEIYSRES ERRKLKKSQM DQVERENNST TNVISFHSSE IWLAMRYSQL QILLYRPSAL 

       610        620        630        640        650        660 
MPKPPIDSLS TLGEFCLQAW KHTYTLYKKR LLPLNWITLF RTLTICNTIL YCLCQWSIDL 

       670        680        690        700        710        720 
IESKIEIQQC VEILRHFGER WIFAMRCADV FQNISNTILD ISLSHGKVPN MDQLTRELFG 

       730        740 
ASDSYQDILD ENNVDVSWVD KLV 

References and documentation are available.

Number of amino acids: 743

Molecular weight: 83473.22

Theoretical pI: 8.78

Amino acid composition: 
Ala (A)  40	  5.4%
Arg (R)  44	  5.9%
Asn (N)  46	  6.2%
Asp (D)  39	  5.2%
Cys (C)  19	  2.6%
Gln (Q)  27	  3.6%
Glu (E)  38	  5.1%
Gly (G)  38	  5.1%
His (H)  14	  1.9%
Ile (I)  48	  6.5%
Leu (L)  72	  9.7%
Lys (K)  45	  6.1%
Met (M)  22	  3.0%
Phe (F)  27	  3.6%
Pro (P)  35	  4.7%
Ser (S)  85	 11.4%
Thr (T)  40	  5.4%
Trp (W)   9	  1.2%
Tyr (Y)  22	  3.0%
Val (V)  33	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 89

Atomic composition:

Carbon      C	      3661
Hydrogen    H	      5826
Nitrogen    N	      1030
Oxygen      O	      1118
Sulfur      S	        41

Formula: C3661H5826N1030O1118S41
Total number of atoms: 11676

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    83405
Abs 0.1% (=1 g/l)   0.999, assuming all pairs of Cys residues form cystines


Ext. coefficient    82280
Abs 0.1% (=1 g/l)   0.986, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.00
This classifies the protein as unstable.



Aliphatic index: 81.25

Grand average of hydropathicity (GRAVY): -0.380