ProtParam
User-provided sequence:
10 20 30 40 50 60
MDGRLPDKGI LKSEALKQYI METTAYPREH ELLKELREAT IQRYGNLSEM GVPVDESLFL
70 80 90 100 110 120
SMLVKIINAK NTIEIGVFTG YSLFTVALAL PEDGRITAID IDQAGYNLGL EFMKKAGVDH
130 140 150 160 170 180
KINFIQSDAV RGLDQLLNGE KQEYDFAFVD ADKTNYVYFL EKLLKLVKVG GIIAFDNTLW
190 200 210 220 230
FGTLIQKENE VPGHMRAYRE ALLEFNKILA RDPRVEIAQI SIGDGLTLCR RLI
References and
documentation are available.
Number of amino acids: 233
Molecular weight: 26350.49
Theoretical pI: 5.25
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1192
Hydrogen H 1900
Nitrogen N 310
Oxygen O 347
Sulfur S 7
Formula: C
1192H
1900N
310O
347S
7
Total number of atoms: 3756
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.718, assuming all pairs of Cys residues form cystines
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.718, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 19.47
This classifies the protein as stable.
Aliphatic index: 107.17
Grand average of hydropathicity (GRAVY): -0.072