ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGVTYQRFP TVKIRELKDD YAKFELRETD VSMANALRRV MISEVPTMAI HLVKIEVNSS 

        70         80         90        100        110        120 
VLNDEFIAQR LSLIPLTSER AMSMRFCQDC EDCNGDEHCE FCSVEFPLSA KCVTDQTLDV 

       130        140        150        160        170        180 
TSRDLYSADP TVTPVDFTSN SSTSDSSEHK GIIIAKLRRG QELKLKALAR KGIGKDHAKW 

       190        200        210        220        230        240 
SPAATVTYMY EPDIIINEEM MNTLTDEEKI DLIESSPTKV FGIDPVTGQV VVVDPEAYTY 

       250        260        270        280        290        300 
DEEVIKKAEA MGKPGLIEIH PKHDSFVFTV ESTGALKASQ LVLNAIDILK QKLDAIRLSD 

       310 
NTVEADDQFG ELGAHMREG 

References and documentation are available.

Number of amino acids: 319

Molecular weight: 35575.41

Theoretical pI: 4.77

Amino acid composition: 
Ala (A)  23	  7.2%
Arg (R)  14	  4.4%
Asn (N)   9	  2.8%
Asp (D)  27	  8.5%
Cys (C)   6	  1.9%
Gln (Q)   9	  2.8%
Glu (E)  28	  8.8%
Gly (G)  14	  4.4%
His (H)   7	  2.2%
Ile (I)  22	  6.9%
Leu (L)  25	  7.8%
Lys (K)  21	  6.6%
Met (M)  11	  3.4%
Phe (F)  11	  3.4%
Pro (P)  13	  4.1%
Ser (S)  24	  7.5%
Thr (T)  22	  6.9%
Trp (W)   1	  0.3%
Tyr (Y)   7	  2.2%
Val (V)  25	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1556
Hydrogen    H	      2493
Nitrogen    N	       415
Oxygen      O	       501
Sulfur      S	        17

Formula: C1556H2493N415O501S17
Total number of atoms: 4982

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16305
Abs 0.1% (=1 g/l)   0.458, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.448, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.19
This classifies the protein as stable.



Aliphatic index: 87.40

Grand average of hydropathicity (GRAVY): -0.272