ProtParam
User-provided sequence:
10 20 30 40 50 60
MDGYEATRIV LSRIQSLDPE NASKIMGLLL LQDHGEKEMI RLAFGPETLV HSVIVKAKKE
70 80 90 100 110 120
LGLMNCSRSP WSHQDELISP KNNRGSSLNP ASLPFYANGG RSSRDLTNDF ELMDDMNSRS
130 140 150 160 170 180
TDFLGSVHAR SGSCVLDGLG YGGDSDLGFG GVPCSYFARG FCKNGASCRF VHSDGGADLV
190 200 210 220 230 240
GSPSRIELLR SNSVPPRLAH HFMTRSSLPS FSTKGVNLQQ NDVQRAAAAL MIGDELQKLG
250 260 270 280 290 300
RWRPERIDLS AMACPASRQI YLTFPADSRF REEDVSNYFS TFGPVQDVRI PYQQKRMFGF
310 320 330 340 350 360
VTFVYPETVK SILAKGNPHF VCDSRVLVKP YKEKGKVPDK YRTNQTTERE LSPTGLDSSP
370 380 390 400 410 420
RDVLGGRGFY NNTQDVLWRS KFEEEILELQ SRRLMNLQLL DVKKHFQLNS PTNIHSPNPF
430 440 450 460 470 480
SQSLISPRPL SVIKREYDGG EKGKGSSKEG SDDDTMNLPE RLEDSLPDSP FASPAHHLLL
490 500 510 520 530 540
FADSADNNGS DLWSPSSDND DNSTPSTLSD SFNSFNYQMP RLPAIGMLPG RGGPTCRVGI
References and
documentation are available.
Number of amino acids: 540
Molecular weight: 59789.16
Theoretical pI: 6.48
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2614
Hydrogen H 4098
Nitrogen N 752
Oxygen O 814
Sulfur S 22
Formula: C
2614H
4098N
752O
814S
22
Total number of atoms: 8300
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41870
Abs 0.1% (=1 g/l) 0.700, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 0.692, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.12
This classifies the protein as unstable.
Aliphatic index: 71.85
Grand average of hydropathicity (GRAVY): -0.530