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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGYEATRIV LSRIQSLDPE NASKIMGLLL LQDHGEKEMI RLAFGPETLV HSVIVKAKKE

70 80 90 100 110 120
LGLMNCSRSP WSHQDELISP KNNRGSSLNP ASLPFYANGG RSSRDLTNDF ELMDDMNSRS

130 140 150 160 170 180
TDFLGSVHAR SGSCVLDGLG YGGDSDLGFG GVPCSYFARG FCKNGASCRF VHSDGGADLV

190 200 210 220 230 240
GSPSRIELLR SNSVPPRLAH HFMTRSSLPS FSTKGVNLQQ NDVQRAAAAL MIGDELQKLG

250 260 270 280 290 300
RWRPERIDLS AMACPASRQI YLTFPADSRF REEDVSNYFS TFGPVQDVRI PYQQKRMFGF

310 320 330 340 350 360
VTFVYPETVK SILAKGNPHF VCDSRVLVKP YKEKGKVPDK YRTNQTTERE LSPTGLDSSP

370 380 390 400 410 420
RDVLGGRGFY NNTQDVLWRS KFEEEILELQ SRRLMNLQLL DVKKHFQLNS PTNIHSPNPF

430 440 450 460 470 480
SQSLISPRPL SVIKREYDGG EKGKGSSKEG SDDDTMNLPE RLEDSLPDSP FASPAHHLLL

490 500 510 520 530 540
FADSADNNGS DLWSPSSDND DNSTPSTLSD SFNSFNYQMP RLPAIGMLPG RGGPTCRVGI


References and documentation are available.

Number of amino acids: 540 Molecular weight: 59789.16 Theoretical pI: 6.48
Amino acid composition: 
Ala (A) 25 4.6% Arg (R) 38 7.0% Asn (N) 28 5.2% Asp (D) 39 7.2% Cys (C) 8 1.5% Gln (Q) 18 3.3% Glu (E) 26 4.8% Gly (G) 44 8.1% His (H) 12 2.2% Ile (I) 18 3.3% Leu (L) 55 10.2% Lys (K) 24 4.4% Met (M) 14 2.6% Phe (F) 26 4.8% Pro (P) 37 6.9% Ser (S) 64 11.9% Thr (T) 20 3.7% Trp (W) 4 0.7% Tyr (Y) 13 2.4% Val (V) 27 5.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 65 Total number of positively charged residues (Arg + Lys): 62 Atomic composition: Carbon C 2614 Hydrogen H 4098 Nitrogen N 752 Oxygen O 814 Sulfur S 22 Formula: C2614H4098N752O814S22 Total number of atoms: 8300 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 41870 Abs 0.1% (=1 g/l) 0.700, assuming all pairs of Cys residues form cystines Ext. coefficient 41370 Abs 0.1% (=1 g/l) 0.692, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 59.12 This classifies the protein as unstable. Aliphatic index: 71.85 Grand average of hydropathicity (GRAVY): -0.530