ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDGYWDVVVS SLQDIYNANE VIPFDDELQT KKLNFLNMSK DQLQLHLNTF QEHMENVNRV 

        70         80         90        100        110        120 
HRILDNVRSN LSLMLNQSRE EKSEENTEDA EEGEGTRMAL SQGKKAVGKV GRSYWTSEYN 

       130        140        150        160        170        180 
PNAPILVGSE VAYKPRRGSA DGEWIQCEVL KVVADGTRFE VRDPEPDELG NSGKVYKCNR 

       190        200        210        220        230        240 
KELLLIPPGF PTKNYPPGTK VLARYPETTT FYPAIVIGTK RDGTCRLRFD GEEEVDKETE 

       250 
VTRRLVLPSP TALANLARK 

References and documentation are available.

Number of amino acids: 259

Molecular weight: 29381.07

Theoretical pI: 5.36

Amino acid composition: 
Ala (A)  13	  5.0%
Arg (R)  18	  6.9%
Asn (N)  17	  6.6%
Asp (D)  15	  5.8%
Cys (C)   3	  1.2%
Gln (Q)   8	  3.1%
Glu (E)  26	 10.0%
Gly (G)  17	  6.6%
His (H)   3	  1.2%
Ile (I)   8	  3.1%
Leu (L)  24	  9.3%
Lys (K)  17	  6.6%
Met (M)   5	  1.9%
Phe (F)   7	  2.7%
Pro (P)  15	  5.8%
Ser (S)  14	  5.4%
Thr (T)  16	  6.2%
Trp (W)   3	  1.2%
Tyr (Y)   9	  3.5%
Val (V)  21	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1288
Hydrogen    H	      2044
Nitrogen    N	       364
Oxygen      O	       406
Sulfur      S	         8

Formula: C1288H2044N364O406S8
Total number of atoms: 4110

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30035
Abs 0.1% (=1 g/l)   1.022, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.018, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.20
This classifies the protein as unstable.



Aliphatic index: 76.72

Grand average of hydropathicity (GRAVY): -0.695