ProtParam
User-provided sequence:
10 20 30 40 50 60
MDHQITTASD FTTTSIPSLY QLDTLLRCHI CKDFLKVPVL TPCGHTFCSL CIRTHLNNQP
70 80 90 100 110 120
NCPLCLFEFR ESLLRSEFLV SEIIQSYTSL RSSLLDALRI PKPTPVPENE EVPGPENSSW
130 140 150 160 170 180
IELISESESD SVNAADDDLQ IVATSERKLA KRSMTDILPL SSKPSKRNFA MFRSERIKKK
190 200 210 220 230 240
SKPNEQMAQC PICQQFYPLK ALEKTHLDEC LTLQSLGKKP KISTTFPTES NPHNKSSSRF
250 260 270 280 290 300
KVRTPEVDKS SCGETSHVDK YLNSMMSAEH QRLPKINFTS MTQSQIKQKL SSLGLSTNGT
310 320 330 340 350 360
RQNMIKRYNH YEMLWNSNFC DSLEPVDEAE LKRQLLSWDV SHNKTPQNSS NKGGISKLMI
370 380 390 400 410 420
MKSNGKSSSY RKLLENFKND KFNRKGWMVM FRKDFARLIR EAKMKIKTGS SDSSGSVGHS
430 440 450 460 470 480
NDGDGVEKVQ SDQGTEDQQM EKDQDTVINE DRVAGERNLP NEDSTDADLS RELMDLNEYS
KDPPGNN
References and
documentation are available.
Number of amino acids: 487
Molecular weight: 55230.36
Theoretical pI: 7.53
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 66
Atomic composition:
Carbon C 2383
Hydrogen H 3824
Nitrogen N 682
Oxygen O 769
Sulfur S 28
Formula: C
2383H
3824N
682O
769S
28
Total number of atoms: 7686
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34670
Abs 0.1% (=1 g/l) 0.628, assuming all pairs of Cys residues form cystines
Ext. coefficient 33920
Abs 0.1% (=1 g/l) 0.614, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.70
This classifies the protein as unstable.
Aliphatic index: 68.25
Grand average of hydropathicity (GRAVY): -0.756