ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDIEAFIHGG SGGGDADADH PLGIFSAADL SGFGFADSST ITGGIPNHIW PQSQNLNARH 

        70         80         90        100        110        120 
PAVSTTIESQ SSICAAASPT SATNLNMKES QTLGGTSGSD SESESLLDIE GGPCEQSTNP 

       130        140        150        160        170        180 
LDVKRVRRMV SNRESARRSR KRKQAHLADL ESQVDQLRGE NASLFKQLTD ANQQFTTSVT 

       190        200        210        220        230        240 
DNRILKSDVE ALRVKVKMAE DMVARGALSC GLGHLGGLSP ALNPRQACRV PDVLAGLDYA 

       250        260        270 
GDDPFTAGLS QPEQLQMPGG EVVDAWGWDN HPNGGMSK 

References and documentation are available.

Number of amino acids: 278

Molecular weight: 29383.48

Theoretical pI: 5.05

Amino acid composition: 
Ala (A)  27	  9.7%
Arg (R)  15	  5.4%
Asn (N)  13	  4.7%
Asp (D)  21	  7.6%
Cys (C)   4	  1.4%
Gln (Q)  15	  5.4%
Glu (E)  14	  5.0%
Gly (G)  29	 10.4%
His (H)   7	  2.5%
Ile (I)  10	  3.6%
Leu (L)  24	  8.6%
Lys (K)   9	  3.2%
Met (M)   7	  2.5%
Phe (F)   7	  2.5%
Pro (P)  14	  5.0%
Ser (S)  30	 10.8%
Thr (T)  14	  5.0%
Trp (W)   3	  1.1%
Tyr (Y)   1	  0.4%
Val (V)  14	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1248
Hydrogen    H	      1993
Nitrogen    N	       377
Oxygen      O	       422
Sulfur      S	        11

Formula: C1248H1993N377O422S11
Total number of atoms: 4051

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18240
Abs 0.1% (=1 g/l)   0.621, assuming all pairs of Cys residues form cystines


Ext. coefficient    17990
Abs 0.1% (=1 g/l)   0.612, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.89
This classifies the protein as unstable.



Aliphatic index: 72.01

Grand average of hydropathicity (GRAVY): -0.471