ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT QIYVSPQQDF 

        70         80         90        100        110        120 
SDLMKSWKNE RCSPELLPYP HQLMKRLLNR ISMQSQLIEN ISMGFLDMQN ASNANPPMPN 

       130        140        150        160        170        180 
ESKLPLLCME TELERLKFVI RSYIRCRLSK IDKFSLYLRQ LNEDENSLIS LTDLLSKDEI 

       190        200        210        220        230        240 
KYHDTHSLIW LKLVNDSILK YMPEELQAIN DTEGSVNMID EPDWNKFVFI HVNGPPDGKW 

       250        260        270        280        290 
NEDPLLQENE FGKPCYTVTI PDLKEEVELT IGSIYVMRYE VIRDLLRDDK VALI 

References and documentation are available.

Number of amino acids: 294

Molecular weight: 33947.69

Theoretical pI: 4.69

Amino acid composition: 
Ala (A)   7	  2.4%
Arg (R)  12	  4.1%
Asn (N)  19	  6.5%
Asp (D)  26	  8.8%
Cys (C)   4	  1.4%
Gln (Q)  11	  3.7%
Glu (E)  23	  7.8%
Gly (G)   8	  2.7%
His (H)   5	  1.7%
Ile (I)  24	  8.2%
Leu (L)  37	 12.6%
Lys (K)  19	  6.5%
Met (M)  11	  3.7%
Phe (F)   7	  2.4%
Pro (P)  15	  5.1%
Ser (S)  25	  8.5%
Thr (T)  15	  5.1%
Trp (W)   4	  1.4%
Tyr (Y)   9	  3.1%
Val (V)  13	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1499
Hydrogen    H	      2387
Nitrogen    N	       393
Oxygen      O	       472
Sulfur      S	        15

Formula: C1499H2387N393O472S15
Total number of atoms: 4766

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35660
Abs 0.1% (=1 g/l)   1.050, assuming all pairs of Cys residues form cystines


Ext. coefficient    35410
Abs 0.1% (=1 g/l)   1.043, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.21
This classifies the protein as unstable.



Aliphatic index: 96.12

Grand average of hydropathicity (GRAVY): -0.433