ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDINSNASVS PRPDGLPMTA GYNSASGKVR NSIRSIINHP EDSARAKERS ETNSPKNNGN 

        70         80         90        100        110        120 
KKPRKKRKTF SCDTCRRVKT RCDFEPFIGK CYRCNVLQLD CSLARNKDNE ILNTLREDGL 

       130        140        150        160        170        180 
LKKINSINHN LGSFSHLNAD SPNESQSSFE KNGTVNFDNY MIDKRLSSLE EHIKSLHQKM 

       190        200        210        220        230        240 
DLIITTAKMS YNSDIKGPGD DIQNVDFSSN KTYDSRLTSG SETIRKTGEY RKENLFLNGF 

       250        260        270        280        290        300 
KLKESPLKLL HDIDERLFPS KATSKAAKLA GQQRPYAVAR VNFLHFYENN QELCHKLAKE 

       310        320        330        340        350        360 
FLVRSHFWII PGGRKEIDVE YAHSHLFITS VFTIIAMSFA DNDKYAAEQE ILYPLVERLL 

       370        380        390        400        410        420 
TNTLTMFEKL TAFDIEAILY CCMFHISRKA KRYRQLKFNS LVLSNFALNS LLHVIDFYQI 

       430        440        450        460        470        480 
KDRVLVKEVY NPEDLYHLRI LNSLTACYLE YSISYGDIRE QDDMLKEFNK LVAKFPQANF 

       490        500        510        520        530        540 
GDDIKISEIN LGDIVNGIFI NLKNYFAQCL DDFNNDRYGG NADTFIFVFP ELNYWLKNWE 

       550        560        570        580        590        600 
ELLAKDGAGV LLFTFDFYHI MICRTFITEF SSTLKSNQRF LKLILNTMKE HSFSLLNGFL 

       610        620        630        640        650        660 
RLPPTLIRGA PIFTCHQLVY ACLTLCDYLY WFDSSERQRV LSLCTKVYWH LSTIGEKMNE 

       670        680        690        700        710        720 
ATDNVGKIIK SIIDTSKTRI NFGSLSKENS DNDKMSTNAN NYTGAGNLHA AKPATSPTNV 

       730        740        750        760        770 
GTLHENLSSS HFMIPDVDQF NSFEDFFQDF FDSLKPNSQK MFTSDKKTEQ TT 

References and documentation are available.

Number of amino acids: 772

Molecular weight: 88647.72

Theoretical pI: 8.15

Amino acid composition: 
Ala (A)  39	  5.1%
Arg (R)  37	  4.8%
Asn (N)  63	  8.2%
Asp (D)  50	  6.5%
Cys (C)  16	  2.1%
Gln (Q)  20	  2.6%
Glu (E)  43	  5.6%
Gly (G)  32	  4.1%
His (H)  21	  2.7%
Ile (I)  51	  6.6%
Leu (L)  79	 10.2%
Lys (K)  60	  7.8%
Met (M)  15	  1.9%
Phe (F)  51	  6.6%
Pro (P)  24	  3.1%
Ser (S)  67	  8.7%
Thr (T)  44	  5.7%
Trp (W)   5	  0.6%
Tyr (Y)  28	  3.6%
Val (V)  27	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3957
Hydrogen    H	      6153
Nitrogen    N	      1073
Oxygen      O	      1181
Sulfur      S	        31

Formula: C3957H6153N1073O1181S31
Total number of atoms: 12395

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70220
Abs 0.1% (=1 g/l)   0.792, assuming all pairs of Cys residues form cystines


Ext. coefficient    69220
Abs 0.1% (=1 g/l)   0.781, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.37
This classifies the protein as stable.



Aliphatic index: 80.87

Grand average of hydropathicity (GRAVY): -0.435