ProtParam
User-provided sequence:
10 20 30 40 50 60
MDKAFDEIIG NSHTDSSSNH KVTRYRRRDL RNELGPRLGF APSDAASRSK DRLYREREEP
70 80 90 100 110 120
PLPKRIRISK IPLDVSDYTL DDMIKEFGSP IFSKIFDNKE DRTCIYEFED PEVLEKIVER
130 140 150 160 170 180
YNGHELHNAK IEVEIYQPQR KHSRMNAHNR RKQTAQEHGR GRPGSHYRQK PNRVSKKNKG
190 200
REKNNTPTSV EALDAELDAY MKG
References and
documentation are available.
Number of amino acids: 203
Molecular weight: 23777.60
Theoretical pI: 9.40
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1027
Hydrogen H 1641
Nitrogen N 323
Oxygen O 319
Sulfur S 5
Formula: C
1027H
1641N
323O
319S
5
Total number of atoms: 3315
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.501, assuming all pairs of Cys residues form cystines
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.501, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.18
This classifies the protein as unstable.
Aliphatic index: 59.11
Grand average of hydropathicity (GRAVY): -1.272