ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKFIQSFSH QYLDSSSSLK LTARRKRKLT ILGLFLFSLI SLMIIISYSN NNILPGLSGI 

        70         80         90        100        110        120 
SISSTFSDYY SNPKQQNKFE QQIQDHQTTK KGKRTIIFPN NFNHVHDHKG SYMMKDSELV 

       130        140        150        160        170        180 
KYYVETMEQA LDPEDLIYRN RFTYKLPNIP YTEQKIEMFS DGGGGGGDTS DSNTDMCPKL 

       190        200        210        220        230        240 
STTIKVEASP AMNKNGDLKK ILKTFLQEDS FYYRELSPFF PDLKKHFDED TIDKHWYQFI 

       250        260        270        280        290        300 
GSTVWLEQYG VHLMVSRIIY TEKDQGSPKF SLAYLQVFDR NWKELDNVEL IVPDPENIST 

       310        320        330        340        350        360 
TNNKNKNKKP YGYKSVLYPT IAPIPVYHNS KQTGGRFYGI EDPRIVLIKT RHGYEEPVLI 

       370        380        390        400        410        420 
YNSHHRKISE KHFDNDQEGK INFNNYRSLF IGWIWQTQLG KIHLEELPNN EFKKNEYIKI 

       430        440        450        460        470        480 
KEFVKPNNNR GRTEKNWALF INYNQRLNQG FDSHVYFANQ LKNLKILKCS ILNDNDDDCE 

       490        500        510        520        530        540 
WEFQMDDYED AGVLHGGTEL ININQLLHQY DYPELNSIKD LIPNGREYWV GFARASLKNC 

       550        560        570        580        590        600 
GCGSRMYRPN LIVLMKDGKN YKFAYVSSFV GLGIEILPWY LDKGLCEHYN LIIPNGISSW 

       610        620        630        640        650        660 
TIEKDLHQKE KDKQVMDYMA FTISRRDATV DVVYVKGLLK ALFTDSSSSK HLLAVEQTGF 

       670        680 
KSVTNVDCAL KNSEKFCKIY GETF 

References and documentation are available.

Number of amino acids: 684

Molecular weight: 79603.41

Theoretical pI: 7.89

Amino acid composition: 
Ala (A)  17	  2.5%
Arg (R)  23	  3.4%
Asn (N)  51	  7.5%
Asp (D)  44	  6.4%
Cys (C)   8	  1.2%
Gln (Q)  28	  4.1%
Glu (E)  40	  5.8%
Gly (G)  40	  5.8%
His (H)  20	  2.9%
Ile (I)  52	  7.6%
Leu (L)  61	  8.9%
Lys (K)  63	  9.2%
Met (M)  14	  2.0%
Phe (F)  36	  5.3%
Pro (P)  26	  3.8%
Ser (S)  51	  7.5%
Thr (T)  32	  4.7%
Trp (W)  10	  1.5%
Tyr (Y)  39	  5.7%
Val (V)  29	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      3600
Hydrogen    H	      5515
Nitrogen    N	       945
Oxygen      O	      1054
Sulfur      S	        22

Formula: C3600H5515N945O1054S22
Total number of atoms: 11136

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   113610
Abs 0.1% (=1 g/l)   1.427, assuming all pairs of Cys residues form cystines


Ext. coefficient   113110
Abs 0.1% (=1 g/l)   1.421, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.44
This classifies the protein as stable.



Aliphatic index: 79.21

Grand average of hydropathicity (GRAVY): -0.583