ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKKKDLLEN EQFLRIQKLN AADAGKRQSI TVDDEGELYG LDTSGNSPAN EHTATTITQN 

        70         80         90        100        110        120 
HSVVASNGDV AFIPGTATEG NTEIVTEEVI ETDDNMFKTH VKTLSSKEKA RYRQGSSNFI 

       130        140        150        160        170        180 
SYFDDMSFEH RPSILDGSVN EPFKTKFVGP TLEKEIRRRE KELMAMRKNL HHRKSSPDAV 

       190        200        210        220        230        240 
DSVGKNDGAA PTTVPTAATS ETVVTVETTI ISSNFSGLYV AFWMAIAFGA VKALIDYYYQ 

       250        260        270        280        290        300 
HNGSFKDSEI LKFMTTNLFT VASVDLLMYL STYFVVGIQY LCKWGVLKWG TTGWIFTSIY 

       310        320        330        340        350        360 
EFLFVIFYMY LTENILKLHW LSKIFLFLHS LVLLMKMHSF AFYNGYLWGI KEELQFSKSA 

       370        380        390        400        410        420 
LAKYKDSIND PKVIGALEKS CEFCSFELSS QSLSDQTQKF PNNISAKSFF WFTMFPTLIY 

       430        440        450        460        470        480 
QIEYPRTKEI RWSYVLEKIC AIFGTIFLMM IDAQILMYPV AMRALAVRNS EWTGILDRLL 

       490        500        510        520        530        540 
KWVGLLVDIV PGFIVMYILD FYLIWDAILN CVAELTRFGD RYFYGDWWNC VSWADFSRIW 

       550        560        570        580        590        600 
NIPVHKFLLR HVYHSSMSSF KLNKSQATLM TFFLSSVVHE LAMYVIFKKL RFYLFFFQML 

       610        620        630        640 
QMPLVALTNT KFMRNRTIIG NVIFWLGICM GPSVMCTLYL TF 

References and documentation are available.

Number of amino acids: 642

Molecular weight: 74022.90

Theoretical pI: 7.59

Amino acid composition: 
Ala (A)  37	  5.8%
Arg (R)  22	  3.4%
Asn (N)  28	  4.4%
Asp (D)  29	  4.5%
Cys (C)   8	  1.2%
Gln (Q)  16	  2.5%
Glu (E)  33	  5.1%
Gly (G)  31	  4.8%
His (H)  14	  2.2%
Ile (I)  45	  7.0%
Leu (L)  64	 10.0%
Lys (K)  41	  6.4%
Met (M)  25	  3.9%
Phe (F)  50	  7.8%
Pro (P)  17	  2.6%
Ser (S)  51	  7.9%
Thr (T)  45	  7.0%
Trp (W)  16	  2.5%
Tyr (Y)  28	  4.4%
Val (V)  42	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      3417
Hydrogen    H	      5199
Nitrogen    N	       837
Oxygen      O	       935
Sulfur      S	        33

Formula: C3417H5199N837O935S33
Total number of atoms: 10421

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   130220
Abs 0.1% (=1 g/l)   1.759, assuming all pairs of Cys residues form cystines


Ext. coefficient   129720
Abs 0.1% (=1 g/l)   1.752, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.20
This classifies the protein as stable.



Aliphatic index: 90.95

Grand average of hydropathicity (GRAVY): 0.092