ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKLSRPEIR QQTTQQMYDV PENILEIDVI NPQTHGIGRN MFTTYEIVCR TNMPYFRLHN 

        70         80         90        100        110        120 
SSVRRRYSEF EKFHDMLERE SGRVSIPPLP GKIFTQRFRD DVIEERRQGL ENFLRLVAGH 

       130        140 
PLIQTHSRVL SSFLQSPEFK PTP 

References and documentation are available.

Number of amino acids: 143

Molecular weight: 16961.34

Theoretical pI: 8.89

Amino acid composition: 
Ala (A)   1	  0.7%
Arg (R)  16	 11.2%
Asn (N)   6	  4.2%
Asp (D)   6	  4.2%
Cys (C)   1	  0.7%
Gln (Q)   9	  6.3%
Glu (E)  12	  8.4%
Gly (G)   6	  4.2%
His (H)   5	  3.5%
Ile (I)  10	  7.0%
Leu (L)  11	  7.7%
Lys (K)   4	  2.8%
Met (M)   5	  3.5%
Phe (F)   9	  6.3%
Pro (P)  11	  7.7%
Ser (S)  10	  7.0%
Thr (T)   9	  6.3%
Trp (W)   0	  0.0%
Tyr (Y)   4	  2.8%
Val (V)   8	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	       750
Hydrogen    H	      1182
Nitrogen    N	       220
Oxygen      O	       218
Sulfur      S	         6

Formula: C750H1182N220O218S6
Total number of atoms: 2376

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.351, assuming all pairs of Cys residues form cystines


Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.351, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 67.60
This classifies the protein as unstable.



Aliphatic index: 74.20

Grand average of hydropathicity (GRAVY): -0.694