ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN VVGARRSSWR 

        70         80         90        100        110        120 
VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL LEKFLIPNAS QPESKVFYLK 

       130        140        150        160        170        180 
MKGDYYRYLA EVAAGDDKKG IVDQSQQAYQ EAFEISKKEM QPTHPIRLGL ALNFSVFYYE 

       190        200        210        220        230        240 
ILNSPEKACS LAKTAFDEAI AELDTLSEES YKDSTLIMQL LRDNLTLWTS DTQGDEAEAG 


EGGEN 

References and documentation are available.

Number of amino acids: 245

Molecular weight: 27771.14

Theoretical pI: 4.73

Amino acid composition: 
Ala (A)  24	  9.8%
Arg (R)  11	  4.5%
Asn (N)  10	  4.1%
Asp (D)  15	  6.1%
Cys (C)   3	  1.2%
Gln (Q)  14	  5.7%
Glu (E)  30	 12.2%
Gly (G)  11	  4.5%
His (H)   1	  0.4%
Ile (I)  10	  4.1%
Leu (L)  24	  9.8%
Lys (K)  20	  8.2%
Met (M)   7	  2.9%
Phe (F)   6	  2.4%
Pro (P)   5	  2.0%
Ser (S)  19	  7.8%
Thr (T)  10	  4.1%
Trp (W)   2	  0.8%
Tyr (Y)  11	  4.5%
Val (V)  12	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1211
Hydrogen    H	      1924
Nitrogen    N	       326
Oxygen      O	       400
Sulfur      S	        10

Formula: C1211H1924N326O400S10
Total number of atoms: 3871

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27515
Abs 0.1% (=1 g/l)   0.991, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.986, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.20
This classifies the protein as unstable.



Aliphatic index: 78.12

Grand average of hydropathicity (GRAVY): -0.635