ProtParam
User-provided sequence:
10 20 30 40 50 60
MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT VAFCDAQSTS
70 80 90 100 110 120
EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS GTDNIDVKAA GELGIAVCNV
130 140 150 160 170 180
PGYGVEEVAD TTMCLILNLY RRTYWLANMV REGKKFTGPE QVREAAHGCA RIRGDTLGLV
190 200 210 220 230 240
GLGRIGSAVA LRAKAFGFNV IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN
250 260 270 280 290 300
EHNHHLINEF TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF
310 320 330 340 350 360
QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC VNKEYFMRTP
370 380 390 400 410 420
PAAAAGGVAA AVYPEGALHH RAHSTTPHDG PHSTTNLGST VGGGPTTVAQ AAAAAVAAAA
430 440 450 460 470
AAALLPSPVP SHLSPQVGGL PLGIVSSQSP LSAPDPNNHL SSSIKTEVKA ESTEAP
References and
documentation are available.
Number of amino acids: 476
Molecular weight: 50737.07
Theoretical pI: 6.35
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2236
Hydrogen H 3585
Nitrogen N 637
Oxygen O 671
Sulfur S 19
Formula: C
2236H
3585N
637O
671S
19
Total number of atoms: 7148
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24910
Abs 0.1% (=1 g/l) 0.491, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.481, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.20
This classifies the protein as stable.
Aliphatic index: 94.16
Grand average of hydropathicity (GRAVY): -0.009