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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT VAFCDAQSTS

70 80 90 100 110 120
EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS GTDNIDVKAA GELGIAVCNV

130 140 150 160 170 180
PGYGVEEVAD TTMCLILNLY RRTYWLANMV REGKKFTGPE QVREAAHGCA RIRGDTLGLV

190 200 210 220 230 240
GLGRIGSAVA LRAKAFGFNV IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN

250 260 270 280 290 300
EHNHHLINEF TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF

310 320 330 340 350 360
QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC VNKEYFMRTP

370 380 390 400 410 420
PAAAAGGVAA AVYPEGALHH RAHSTTPHDG PHSTTNLGST VGGGPTTVAQ AAAAAVAAAA

430 440 450 460 470
AAALLPSPVP SHLSPQVGGL PLGIVSSQSP LSAPDPNNHL SSSIKTEVKA ESTEAP


References and documentation are available.

Number of amino acids: 476 Molecular weight: 50737.07 Theoretical pI: 6.35
Amino acid composition: 
Ala (A) 62 13.0% Arg (R) 25 5.3% Asn (N) 22 4.6% Asp (D) 24 5.0% Cys (C) 9 1.9% Gln (Q) 11 2.3% Glu (E) 25 5.3% Gly (G) 38 8.0% His (H) 16 3.4% Ile (I) 25 5.3% Leu (L) 48 10.1% Lys (K) 19 4.0% Met (M) 10 2.1% Phe (F) 14 2.9% Pro (P) 28 5.9% Ser (S) 25 5.3% Thr (T) 29 6.1% Trp (W) 2 0.4% Tyr (Y) 9 1.9% Val (V) 35 7.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 49 Total number of positively charged residues (Arg + Lys): 44 Atomic composition: Carbon C 2236 Hydrogen H 3585 Nitrogen N 637 Oxygen O 671 Sulfur S 19 Formula: C2236H3585N637O671S19 Total number of atoms: 7148 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 24910 Abs 0.1% (=1 g/l) 0.491, assuming all pairs of Cys residues form cystines Ext. coefficient 24410 Abs 0.1% (=1 g/l) 0.481, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 39.20 This classifies the protein as stable. Aliphatic index: 94.16 Grand average of hydropathicity (GRAVY): -0.009