ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKNTLKRRS RLLQKNEARM SMLLGRNLDD EISKEKKESN KNDKHKKNDQ HKKNDKSNQN 

        70         80         90        100        110        120 
GEDNQNGEDN QNDESNQNDE HKKNDEHKKN DEHNQNDEHN QNDEHNQNDE HNQNDEHNQN 

       130        140        150        160        170        180 
DEHNQNDEHN QNDEHNQNDE SNQNDKNRKE IPPKEEKDKE NNPSVVMENN NNIRKDQDNK 

       190        200        210        220        230        240 
TSEHKSSTNI YNKDKNNDYN KLLDKDDNNN NNKNINKNDE NDFTSINNDI PNKNKITSQF 

       250        260        270        280        290        300 
IITKHEKLHF IILIILCIFI SIFKVYYNNK NNLIYKKKKK GNNNLNIVQM IFFNFINSPN 

       310        320        330        340        350        360 
FFFSFSVFYN ILFLLIIMLM YIKKNNITRK RIQDFFVNMK NKLNNQNEHV FYFINNAVLC 

       370        380        390        400 
ILFMGRIFKS YIISMFLINL FHDILHNYLI GVSMLQPQKV VLL 

References and documentation are available.

Number of amino acids: 403

Molecular weight: 48273.54

Theoretical pI: 6.82

Amino acid composition: 
Ala (A)   2	  0.5%
Arg (R)  10	  2.5%
Asn (N)  84	 20.8%
Asp (D)  34	  8.4%
Cys (C)   2	  0.5%
Gln (Q)  22	  5.5%
Glu (E)  27	  6.7%
Gly (G)   6	  1.5%
His (H)  18	  4.5%
Ile (I)  35	  8.7%
Leu (L)  27	  6.7%
Lys (K)  49	 12.2%
Met (M)  11	  2.7%
Phe (F)  22	  5.5%
Pro (P)   6	  1.5%
Ser (S)  20	  5.0%
Thr (T)   7	  1.7%
Trp (W)   0	  0.0%
Tyr (Y)  10	  2.5%
Val (V)  11	  2.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2091
Hydrogen    H	      3272
Nitrogen    N	       624
Oxygen      O	       669
Sulfur      S	        13

Formula: C2091H3272N624O669S13
Total number of atoms: 6669

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15025
Abs 0.1% (=1 g/l)   0.311, assuming all pairs of Cys residues form cystines


Ext. coefficient    14900
Abs 0.1% (=1 g/l)   0.309, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.62
This classifies the protein as stable.



Aliphatic index: 68.41

Grand average of hydropathicity (GRAVY): -1.307