ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDKYTALIHD ENFSTLTLNV SRYPKSLAYW EKLLNYIVKA SAPICKSTEP QLLKLIRCTY 

        70         80         90        100        110        120 
SSMLNEFPYL ENYYIDFALL EYKLGNVSMS HKIFQRGLQA FNQRSLLLWT SYLKFCNNVI 

       130        140        150        160        170        180 
SHQKQLFKKY ETAEEYVGLH FFSGEFWDLY LEQISSRCTS SKKYWNVLRK ILEIPLHSFS 

       190        200        210        220        230        240 
KFYALWLQRI DDIMDLKQLS QLTSKDELLK KLKIDINYSG RKGPYLQDAK KKLKKITKEM 

       250        260        270        280        290        300 
YMVVQYQVLE IYSIFESKIY INYYTSPETL VSSDEIETWI KYLDYTITLQ TDSLTHLNFQ 

       310        320        330        340        350        360 
RALLPLAHYD LVWIKYSKWL INSKNDLLGA KNVLLMGLKF SLKKTEIIKL LYSVICKLNE 

       370        380        390        400        410        420 
YVLLRNLLEK IESSYSDNVE NVDDFEIFWD YLQFKTFCQN SLYSSRYSDS QSNGLLNKEL 

       430        440        450        460        470        480 
FDKVWKRLSC KEKKSGQEIL LNNLVQFYSK DTVEFVEKNI FQKIIEFGWE YYLQNGMFWN 

       490        500        510        520        530        540 
CYCRLIYFDT SRSYLDKRQY IVRKIWPQID KKFAQSVLPS LTEFCESYFP EEMDTLEEMF 


TEEP 

References and documentation are available.

Number of amino acids: 544

Molecular weight: 65145.27

Theoretical pI: 8.24

Amino acid composition: 
Ala (A)  13	  2.4%
Arg (R)  16	  2.9%
Asn (N)  28	  5.1%
Asp (D)  26	  4.8%
Cys (C)  10	  1.8%
Gln (Q)  25	  4.6%
Glu (E)  40	  7.4%
Gly (G)  12	  2.2%
His (H)   7	  1.3%
Ile (I)  37	  6.8%
Leu (L)  75	 13.8%
Lys (K)  54	  9.9%
Met (M)  10	  1.8%
Phe (F)  30	  5.5%
Pro (P)  12	  2.2%
Ser (S)  49	  9.0%
Thr (T)  24	  4.4%
Trp (W)  13	  2.4%
Tyr (Y)  41	  7.5%
Val (V)  22	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3013
Hydrogen    H	      4606
Nitrogen    N	       726
Oxygen      O	       844
Sulfur      S	        20

Formula: C3013H4606N726O844S20
Total number of atoms: 9209

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   133215
Abs 0.1% (=1 g/l)   2.045, assuming all pairs of Cys residues form cystines


Ext. coefficient   132590
Abs 0.1% (=1 g/l)   2.035, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.12
This classifies the protein as unstable.



Aliphatic index: 94.41

Grand average of hydropathicity (GRAVY): -0.315