ProtParam
User-provided sequence:
10 20 30 40 50 60
MDKYTNRDHP DYIPGTFNIY SSQNLENGII YESKLKKTSS GVVLIPQPSY SPNDPLNWSS
70 80 90 100 110 120
WRKLAHFGLM AFITAFTAAT SNDAGAAQDS LNEIYGISYD SMNTGAGVLF LGIGWSTLFL
130 140 150 160 170 180
APFANLYGRK ITYIVCTTLG LFGALWFALA KRTSDTIWSQ LFVGISESCA EAQVQLSLSD
190 200 210 220 230 240
IFFQHQLGSV LTVYIMCTSI GTFLGPLIAG YISAFTNFRW VGWVAVIISG GLLITIIFGC
250 260 270 280 290 300
EETYFDRGQY MTPLTSCQSG YEDGTTLQNS DNTAVSRRKR HLDAKLSTPG AMGEKGVDLS
310 320 330 340 350 360
ETAEFEVNNE EEVTIPETRE LIDGSKEHLK PYPKRVAILT KATNLKGYGF KQYFKYLKIN
370 380 390 400 410 420
LRMFLFPPVW LSGMFWGIQD VFLTFYLTTQ ESAYYEPPWN YSDFGVAIMN VPTLIGAVIG
430 440 450 460 470 480
CICAGIVSDY FVLWMARHNR GILEAEFRLY FSIATAIIGP AGLLMFGIGT ARQWPWQAIY
490 500 510 520 530 540
VGLGFVGFAW GCSGDIAMAY LMDCYPDMVL EGMVCTAIIN NTISCIFTFT CSDWLAASGT
550 560 570 580
ENTYIALAVI NFGITAFALP MYYYGKRIRL WTKRWYLQSV NLRDGV
References and
documentation are available.
Number of amino acids: 586
Molecular weight: 65348.20
Theoretical pI: 5.53
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 3007
Hydrogen H 4535
Nitrogen N 737
Oxygen O 840
Sulfur S 28
Formula: C
3007H
4535N
737O
840S
28
Total number of atoms: 9147
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 141930
Abs 0.1% (=1 g/l) 2.172, assuming all pairs of Cys residues form cystines
Ext. coefficient 141180
Abs 0.1% (=1 g/l) 2.160, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.15
This classifies the protein as stable.
Aliphatic index: 91.25
Grand average of hydropathicity (GRAVY): 0.204