ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLFKRKVKE WVYSLSTDDH YAEYNPDETP TFNMGKRLNS NNGQVNPSQM HLNSVDEEMS 

        70         80         90        100        110        120 
MGFQNGVPSN EDINIDEFTS TESNDGVSET LLAWRHIDFW TSEHNPDLNA TLSDPCTQND 

       130        140        150        160        170        180 
ITHAEEDLEV SFPNPVKASF KIHDGQEDLE SMTGTSGLFY GFQLMTLDQV VAMTQAWRNV 

       190        200        210        220        230        240 
AKNLNKRSQQ GLSHVTSTGS SSSMERLNGN KFKLPNIPDQ KSIPPNAVQP VYAHPAWIPL 

       250        260        270        280        290        300 
ITDNAGNHIG VDLAPGPNGK YAQIITFGRD FDTKFVIAEN WGEFLLSFAN DLEAGNWYLV 

       310        320        330        340        350        360 
DDNDDYFSGD GELVFRDKKS NGPIQDYFEV LKRRTWIKYQ ENLRSQQQKS QPDTSLQEQK 

       370        380        390        400        410        420 
YVPASQKKVA AEQPSTLNAE SIKGEDSGSA DVQSVQDHES VKIVKTEPSE AETTTVNTES 

       430        440        450        460        470        480 
LGQAEHEIKA DNVDIKQESE RKEDEKQPKV EEKEHVENEH VTESAKKDDD VNKQTEEMNK 

       490        500 
KEENEIRSDD AKVEEAREEF ENIAL 

References and documentation are available.

Number of amino acids: 505

Molecular weight: 57079.37

Theoretical pI: 4.59

Amino acid composition: 
Ala (A)  29	  5.7%
Arg (R)  14	  2.8%
Asn (N)  39	  7.7%
Asp (D)  41	  8.1%
Cys (C)   1	  0.2%
Gln (Q)  29	  5.7%
Glu (E)  53	 10.5%
Gly (G)  25	  5.0%
His (H)  13	  2.6%
Ile (I)  21	  4.2%
Leu (L)  30	  5.9%
Lys (K)  36	  7.1%
Met (M)  10	  2.0%
Phe (F)  19	  3.8%
Pro (P)  23	  4.6%
Ser (S)  42	  8.3%
Thr (T)  29	  5.7%
Trp (W)   8	  1.6%
Tyr (Y)  11	  2.2%
Val (V)  32	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2479
Hydrogen    H	      3823
Nitrogen    N	       685
Oxygen      O	       844
Sulfur      S	        11

Formula: C2479H3823N685O844S11
Total number of atoms: 7842

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.058, assuming all pairs of Cys residues form cystines


Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.058, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.92
This classifies the protein as unstable.



Aliphatic index: 63.50

Grand average of hydropathicity (GRAVY): -0.920