ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLKKPSARG GDDKQQIVGD EHSRRNKLSE EDKVRSPICC MMGHIDTGKT KLLDYITGNV 

        70         80         90        100        110        120 
QEGEAGGTTQ KMGATYLSAR NILEKTMELK SDTKLKVPRP LFIDTPGYEF YTNLRSRGLG 

       130        140        150        160        170        180 
LCDFAILVVD IMHGLEPQTI ECLNLLRMKN TEFIIALNKV DRLYGWRICE NAPILEAMKQ 

       190        200        210        220        230        240 
QTKDVINEFN MRLNEIINQF KEQGLNSELY YKNKEMGETF SIVPTCAISG EGIPDLLLLL 

       250        260        270        280        290        300 
VQLTQKTMVE KLITYVDKVQ QCTVLEVKVM EGYGTTIDVV LVNGELHEGG QIVVCGLQGP 

       310        320        330        340        350        360 
IVTTIRALLT PHPIKELHVN GNHVHHEVIK AAECINIIAK DLEHVIVGTA LHVVGPDDDI 

       370        380        390        400        410        420 
EAIKELVMED VNSVLSRIDK SGEGVYIQAS TLGSLEALLE FLKSPAVKLP VGGIGIGPVQ 

       430        440        450        460        470        480 
KKDVMKAGVM LERKKEFATI LALDVEVTTE ARELADEMEV KIFCSDIMYH LFDQYQAYIC 


G 

References and documentation are available.

Number of amino acids: 481

Molecular weight: 53516.13

Theoretical pI: 5.28

Amino acid composition: 
Ala (A)  24	  5.0%
Arg (R)  16	  3.3%
Asn (N)  19	  4.0%
Asp (D)  27	  5.6%
Cys (C)  11	  2.3%
Gln (Q)  19	  4.0%
Glu (E)  42	  8.7%
Gly (G)  38	  7.9%
His (H)  12	  2.5%
Ile (I)  40	  8.3%
Leu (L)  52	 10.8%
Lys (K)  36	  7.5%
Met (M)  17	  3.5%
Phe (F)  11	  2.3%
Pro (P)  16	  3.3%
Ser (S)  17	  3.5%
Thr (T)  29	  6.0%
Trp (W)   1	  0.2%
Tyr (Y)  13	  2.7%
Val (V)  41	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2370
Hydrogen    H	      3854
Nitrogen    N	       628
Oxygen      O	       717
Sulfur      S	        28

Formula: C2370H3854N628O717S28
Total number of atoms: 7597

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25495
Abs 0.1% (=1 g/l)   0.476, assuming all pairs of Cys residues form cystines


Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.465, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.19
This classifies the protein as stable.



Aliphatic index: 104.30

Grand average of hydropathicity (GRAVY): -0.071