ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLLGDIVEK DTSDSVESND NGTLSTNNCG TGFPELYKPK KISSWKERLR EKRAQKKKTS 

        70         80         90        100        110        120 
GKDAEKQQTS TDAPLSEAKS IHNENIKVLQ GMSDEQIVQE REDLYNSLDP KLIAKLLKNI 

       130        140        150        160        170        180 
NKRAKDENNT PLFAEIEGAS GTWVGGNKQG IYDLPPLDDE DVDVALEIRP MLGKDAKHVQ 

       190        200        210        220        230        240 
FEEAGKEKDV EGEGKTNDDV DDIAPLDFQM AQCIDHMKNE ELFKDVHFIK EESQNEINLE 

       250        260        270        280        290        300 
KLDINDPNFN DKLHEKYFPD LPKEVDKLKW MQPVQQKTDK NYIIEDVSEC RFDFNGDLVP 

       310        320        330        340        350        360 
PTRQIDSTIH SGLHHHSDSP ELAGYTIVEL EHLARSTFPS QRCIAIQTLG RILYKLGQKS 

       370        380        390        400        410        420 
YYQLVPEIDA DTYKEDGSIS NVMDKIYSMF WDLIKDGKVI ESLEISSDEK FTRNLSVRNY 

       430 
AIDALWLWKQ GGGDFRTKK 

References and documentation are available.

Number of amino acids: 439

Molecular weight: 50106.09

Theoretical pI: 4.90

Amino acid composition: 
Ala (A)  19	  4.3%
Arg (R)  14	  3.2%
Asn (N)  24	  5.5%
Asp (D)  45	 10.3%
Cys (C)   4	  0.9%
Gln (Q)  20	  4.6%
Glu (E)  39	  8.9%
Gly (G)  25	  5.7%
His (H)  10	  2.3%
Ile (I)  30	  6.8%
Leu (L)  38	  8.7%
Lys (K)  45	 10.3%
Met (M)   8	  1.8%
Phe (F)  14	  3.2%
Pro (P)  18	  4.1%
Ser (S)  29	  6.6%
Thr (T)  19	  4.3%
Trp (W)   6	  1.4%
Tyr (Y)  12	  2.7%
Val (V)  20	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2205
Hydrogen    H	      3470
Nitrogen    N	       596
Oxygen      O	       712
Sulfur      S	        12

Formula: C2205H3470N596O712S12
Total number of atoms: 6995

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51130
Abs 0.1% (=1 g/l)   1.020, assuming all pairs of Cys residues form cystines


Ext. coefficient    50880
Abs 0.1% (=1 g/l)   1.015, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.77
This classifies the protein as stable.



Aliphatic index: 77.95

Grand average of hydropathicity (GRAVY): -0.804