ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLLKFSSLA ISEINFLHES SFDSIDHSWF LLIGCKLDQD DEIYIPINGN EAESQWYIEK 

        70         80         90        100        110        120 
VIRIPMQEND KINQERLERR INLTKVTQKD ICILGILDLC QLEEDENITN KVTEKVLTQL 

       130        140        150        160        170        180 
TALALKYLIK YNVFRQHTSF QEAVNSLKGY KIENSVQIGA EIILDFLQDK VQIKDVNDRY 

       190        200        210        220        230        240 
QIPTPNNTVD PGFDEFQLID MKDKEINIQK YNNNTIRKLL EKINRMIIFL KNYDATDKPF 

       250        260        270        280        290 
SSTQDVILRK ISMLVTQLQR GGTSDMNYLL DNKINEIKLL EISCKQWEIS NMLKK 

References and documentation are available.

Number of amino acids: 295

Molecular weight: 34519.69

Theoretical pI: 5.15

Amino acid composition: 
Ala (A)   7	  2.4%
Arg (R)  10	  3.4%
Asn (N)  24	  8.1%
Asp (D)  23	  7.8%
Cys (C)   4	  1.4%
Gln (Q)  19	  6.4%
Glu (E)  22	  7.5%
Gly (G)   8	  2.7%
His (H)   3	  1.0%
Ile (I)  36	 12.2%
Leu (L)  33	 11.2%
Lys (K)  27	  9.2%
Met (M)   7	  2.4%
Phe (F)  11	  3.7%
Pro (P)   6	  2.0%
Ser (S)  17	  5.8%
Thr (T)  14	  4.7%
Trp (W)   3	  1.0%
Tyr (Y)   9	  3.1%
Val (V)  12	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1541
Hydrogen    H	      2476
Nitrogen    N	       404
Oxygen      O	       469
Sulfur      S	        11

Formula: C1541H2476N404O469S11
Total number of atoms: 4901

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30160
Abs 0.1% (=1 g/l)   0.874, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.866, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.04
This classifies the protein as stable.



Aliphatic index: 105.39

Grand average of hydropathicity (GRAVY): -0.386