ProtParam
User-provided sequence:
10 20 30 40 50 60
MDLPKDKSDR THQRINLNNS GTDRTNDLYL HIVQTFGCIE TTATENATKL LMLGDVEVEI
70 80 90 100 110 120
SASSVSIEWT QKSMISQTIA DSIVIMIIGL CASDKNVLSE SELKERNHNV WKIQELQNLF
130 140 150 160 170 180
REQFGDSFSI DEGIGKKENV KNGSVTIGKS KATIDFSTMK LIDCNSNPLK GRVESILSIG
QKLTTPLC
References and
documentation are available.
Number of amino acids: 188
Molecular weight: 20865.74
Theoretical pI: 5.44
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 903
Hydrogen H 1482
Nitrogen N 250
Oxygen O 296
Sulfur S 9
Formula: C
903H
1482N
250O
296S
9
Total number of atoms: 2940
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12740
Abs 0.1% (=1 g/l) 0.611, assuming all pairs of Cys residues form cystines
Ext. coefficient 12490
Abs 0.1% (=1 g/l) 0.599, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 22.42
This classifies the protein as stable.
Aliphatic index: 93.30
Grand average of hydropathicity (GRAVY): -0.311