ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLQRGFVFL SLVLSFMIIE TTAYRERQLL LLQPPQETAI DTANAVVTVQ DRGLKTRRPE 

        70         80         90        100        110        120 
HKNAYATMMY MGTPRDYEFY VATRVLIRSL RSLHVEADLV VIASLDVPLR WVQTLEEEDG 

       130        140        150        160        170        180 
AKVVRVENVD NPYRRQTNFN SRFKLTLNKL YAWALSDYDR VVMLDADNLF LKKADELFQC 

       190        200        210        220        230        240 
GRFCAVFINP CIFHTGLFVL QPSVEVFKDM LHELQVGRKN PDGADQGFLV SYFSDLLDQP 

       250        260        270        280        290        300 
LFSPPSNGSV LNGHLRLPLG YQMDASYFYL KLRWNIPCGP NSVITFPGAV WLKPWYWWSW 

       310        320        330        340        350        360 
PVLPLGFSWH EQRRATIGYS AEMPLVIIQA MFYLGIIVVT RLARPNITKL CYRRSDRNLT 

       370        380        390        400        410        420 
TIQAGFKLIA LLSVVAAYIF PFFTIPHTIH PLIGWSLYLM ASFALSSISI NTLLLPTLPV 

       430        440        450        460        470        480 
LTPWLGILGT LLVMAFPWYP DGVVRALSVF AYAFCCAPFV WVSFRKITSH LQVLIEKEVL 

       490 
FPRLGDSGVT SGFSKLY 

References and documentation are available.

Number of amino acids: 497

Molecular weight: 56798.46

Theoretical pI: 8.92

Amino acid composition: 
Ala (A)  33	  6.6%
Arg (R)  30	  6.0%
Asn (N)  17	  3.4%
Asp (D)  22	  4.4%
Cys (C)   7	  1.4%
Gln (Q)  17	  3.4%
Glu (E)  17	  3.4%
Gly (G)  25	  5.0%
His (H)   9	  1.8%
Ile (I)  26	  5.2%
Leu (L)  68	 13.7%
Lys (K)  16	  3.2%
Met (M)  12	  2.4%
Phe (F)  32	  6.4%
Pro (P)  31	  6.2%
Ser (S)  31	  6.2%
Thr (T)  27	  5.4%
Trp (W)  13	  2.6%
Tyr (Y)  21	  4.2%
Val (V)  43	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2641
Hydrogen    H	      4056
Nitrogen    N	       668
Oxygen      O	       689
Sulfur      S	        19

Formula: C2641H4056N668O689S19
Total number of atoms: 8073

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   103165
Abs 0.1% (=1 g/l)   1.816, assuming all pairs of Cys residues form cystines


Ext. coefficient   102790
Abs 0.1% (=1 g/l)   1.810, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.10
This classifies the protein as unstable.



Aliphatic index: 105.49

Grand average of hydropathicity (GRAVY): 0.244