ProtParam
User-provided sequence:
10 20 30 40 50 60
MDLSPVKEAL AAKSFDKIAD ICDTLMLQVA SEGIEYHDDW PYAIHLLGYF YVDDCDSARF
70 80 90 100 110 120
LWKRIPTAIK ERKPEVVAAW GIGQKLWTHD YAGVYEAIRG YDWSQEAKDM VAAFSDLYTK
130 140 150 160 170 180
RMFQLLLSAY STITIHDLAL FLGMTEDDAT TYVVENGWTV DAASQMASVK KQAVKREQKV
190
DSSKLQRLTE YVFHLEH
References and
documentation are available.
Number of amino acids: 197
Molecular weight: 22546.66
Theoretical pI: 5.10
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 1022
Hydrogen H 1559
Nitrogen N 259
Oxygen O 301
Sulfur S 8
Formula: C
1022H
1559N
259O
301S
8
Total number of atoms: 3149
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49515
Abs 0.1% (=1 g/l) 2.196, assuming all pairs of Cys residues form cystines
Ext. coefficient 49390
Abs 0.1% (=1 g/l) 2.191, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.76
This classifies the protein as stable.
Aliphatic index: 87.21
Grand average of hydropathicity (GRAVY): -0.206