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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLSPVKEAL AAKSFDKIAD ICDTLMLQVA SEGIEYHDDW PYAIHLLGYF YVDDCDSARF

70 80 90 100 110 120
LWKRIPTAIK ERKPEVVAAW GIGQKLWTHD YAGVYEAIRG YDWSQEAKDM VAAFSDLYTK

130 140 150 160 170 180
RMFQLLLSAY STITIHDLAL FLGMTEDDAT TYVVENGWTV DAASQMASVK KQAVKREQKV

190
DSSKLQRLTE YVFHLEH


References and documentation are available.

Number of amino acids: 197 Molecular weight: 22546.66 Theoretical pI: 5.10
Amino acid composition: 
Ala (A) 22 11.2% Arg (R) 7 3.6% Asn (N) 1 0.5% Asp (D) 18 9.1% Cys (C) 2 1.0% Gln (Q) 8 4.1% Glu (E) 12 6.1% Gly (G) 8 4.1% His (H) 6 3.0% Ile (I) 10 5.1% Leu (L) 18 9.1% Lys (K) 14 7.1% Met (M) 6 3.0% Phe (F) 7 3.6% Pro (P) 4 2.0% Ser (S) 12 6.1% Thr (T) 11 5.6% Trp (W) 6 3.0% Tyr (Y) 11 5.6% Val (V) 14 7.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 30 Total number of positively charged residues (Arg + Lys): 21 Atomic composition: Carbon C 1022 Hydrogen H 1559 Nitrogen N 259 Oxygen O 301 Sulfur S 8 Formula: C1022H1559N259O301S8 Total number of atoms: 3149 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 49515 Abs 0.1% (=1 g/l) 2.196, assuming all pairs of Cys residues form cystines Ext. coefficient 49390 Abs 0.1% (=1 g/l) 2.191, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 35.76 This classifies the protein as stable. Aliphatic index: 87.21 Grand average of hydropathicity (GRAVY): -0.206