ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLSSRLSSG SSRIPKRHRD YRDEEPRRER GSGGIGREDP RGHYGSERPR RRRRDESDFR 

        70         80         90        100        110        120 
RHRESRERSY REDERPRRER RYDDYEPRSL RYSSVGRSRS PPPSRERSVR SIEQELEQLR 

       130        140        150        160        170        180 
DVTPINQWKR KRSLWDIKPP GYELVTADQA KMSGVFPLPG APRAAVTDPE KLLEFARSAE 

       190        200        210        220        230        240 
GSIIAPPPPL QPGASRQARR LVVTGIPNEF VEDAFVSFIE DLFISTTYHK PETKHFSSVN 

       250        260        270        280        290        300 
VCKEENFAIL EVATPEDATF LWGLQSESYS NDVFLKFQRI QNYIVPQITP EVSQKRSDDY 

       310        320        330        340        350        360 
AKNDVLDSKD KIYISNLPLN LGEDQVVELL KPFGDLLSFQ LIKNIADGSS KGFCFCEFKN 

       370        380        390        400        410        420 
PSDAEVAISG LDGKDTYGNK LHAQFACVGL NQAMIDKSNG MAILTELAKA SSQSIPTRVL 

       430        440        450        460        470        480 
QLHNLITGDE IMDVQEYEDI YESVKTQFSN YGPLIDIKIP RSIGTRNSGL GTGKVFVRYS 

       490        500        510 
DIRSAEVAME EMKGCKFNDR TIVIAFYGED CYKANAW 

References and documentation are available.

Number of amino acids: 517

Molecular weight: 58933.16

Theoretical pI: 6.72

Amino acid composition: 
Ala (A)  29	  5.6%
Arg (R)  50	  9.7%
Asn (N)  19	  3.7%
Asp (D)  36	  7.0%
Cys (C)   6	  1.2%
Gln (Q)  19	  3.7%
Glu (E)  43	  8.3%
Gly (G)  32	  6.2%
His (H)   7	  1.4%
Ile (I)  32	  6.2%
Leu (L)  36	  7.0%
Lys (K)  28	  5.4%
Met (M)   7	  1.4%
Phe (F)  22	  4.3%
Pro (P)  32	  6.2%
Ser (S)  50	  9.7%
Thr (T)  18	  3.5%
Trp (W)   4	  0.8%
Tyr (Y)  19	  3.7%
Val (V)  28	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      2587
Hydrogen    H	      4077
Nitrogen    N	       751
Oxygen      O	       801
Sulfur      S	        13

Formula: C2587H4077N751O801S13
Total number of atoms: 8229

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50685
Abs 0.1% (=1 g/l)   0.860, assuming all pairs of Cys residues form cystines


Ext. coefficient    50310
Abs 0.1% (=1 g/l)   0.854, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 60.41
This classifies the protein as unstable.



Aliphatic index: 72.61

Grand average of hydropathicity (GRAVY): -0.717