ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDLVNHLTDR LLFAIPKKGR LYSKSVSILN GADITFHRSQ RLDIALSTSL PVALVFLPAA 

        70         80         90        100        110        120 
DIPTFVGEGK CDLGITGVDQ VRESNVDVDL AIDLQFGNCK LQVQVPVNGE YKKPEQLIGK 

       130        140        150        160        170        180 
TIVTSFVKLA EKYFADLEGT TVEKMTTRIK FVSGSVEASC ALGIGDAIVD LVESGETMRA 

       190        200        210        220        230        240 
AGLVDIATVL STSAYLIESK NPKSDKSLIA TIKSRIEGVM TAQRFVSCIY NAPEDKLPEL 

       250        260        270        280        290 
LKVTPGRRAP TISKIDDEGW VAVSSMIERK TKGVVLDELK RLGASDIMVF EISNCRV 

References and documentation are available.

Number of amino acids: 297

Molecular weight: 32266.41

Theoretical pI: 5.87

Amino acid composition: 
Ala (A)  22	  7.4%
Arg (R)  14	  4.7%
Asn (N)   8	  2.7%
Asp (D)  20	  6.7%
Cys (C)   5	  1.7%
Gln (Q)   7	  2.4%
Glu (E)  18	  6.1%
Gly (G)  20	  6.7%
His (H)   2	  0.7%
Ile (I)  23	  7.7%
Leu (L)  30	 10.1%
Lys (K)  22	  7.4%
Met (M)   6	  2.0%
Phe (F)  10	  3.4%
Pro (P)  11	  3.7%
Ser (S)  24	  8.1%
Thr (T)  19	  6.4%
Trp (W)   1	  0.3%
Tyr (Y)   5	  1.7%
Val (V)  30	 10.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1433
Hydrogen    H	      2355
Nitrogen    N	       381
Oxygen      O	       437
Sulfur      S	        11

Formula: C1433H2355N381O437S11
Total number of atoms: 4617

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13200
Abs 0.1% (=1 g/l)   0.409, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.401, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.65
This classifies the protein as stable.



Aliphatic index: 106.30

Grand average of hydropathicity (GRAVY): 0.097