ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDMEIDDPMH GSDEDQLSPT LDEDMNSDDG KNNTKARSND EDTDEELEDF NFKPGSSGIA 

        70         80         90        100        110        120 
DHKSSKPLKL TNTNINQLDQ WIEHLSKCEP LSEDDVARLC KMAVDVLQFE ENVKPINVPV 

       130        140        150        160        170        180 
TICGDVHGQF HDLLELFKIG GPCPDTNYLF MGDYVDRGYY SVETVSYLVA MKVRYPHRIT 

       190        200        210        220        230        240 
ILRGNHESRQ ITQVYGFYDE CLRKYGSANV WKMFTDLFDY FPVTALVDNK IFCLHGGLSP 

       250        260        270        280        290        300 
MIETIDQVRD LNRIQEVPHE GPMCDLLWSD PDDRGGWGIS PRGAGFTFGQ DISEQFNHTN 

       310        320        330        340        350        360 
DLSLIARAHQ LVMEGYSWSH QQNVVTIFSA PNYCYRCGNQ AAIMEVDENH NRQFLQYDPS 

       370 
VRPGEPTVTR KTPDYFL 

References and documentation are available.

Number of amino acids: 377

Molecular weight: 43046.96

Theoretical pI: 4.63

Amino acid composition: 
Ala (A)  13	  3.4%
Arg (R)  17	  4.5%
Asn (N)  22	  5.8%
Asp (D)  38	 10.1%
Cys (C)   9	  2.4%
Gln (Q)  17	  4.5%
Glu (E)  24	  6.4%
Gly (G)  25	  6.6%
His (H)  13	  3.4%
Ile (I)  19	  5.0%
Leu (L)  29	  7.7%
Lys (K)  15	  4.0%
Met (M)  12	  3.2%
Phe (F)  17	  4.5%
Pro (P)  21	  5.6%
Ser (S)  23	  6.1%
Thr (T)  19	  5.0%
Trp (W)   5	  1.3%
Tyr (Y)  15	  4.0%
Val (V)  24	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1892
Hydrogen    H	      2873
Nitrogen    N	       513
Oxygen      O	       598
Sulfur      S	        21

Formula: C1892H2873N513O598S21
Total number of atoms: 5897

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50350
Abs 0.1% (=1 g/l)   1.170, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   1.158, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.78
This classifies the protein as stable.



Aliphatic index: 71.56

Grand average of hydropathicity (GRAVY): -0.575