ProtParam
User-provided sequence:
10 20 30 40 50 60
MDMKGTYLVT VILLVSTLSV GMCSNGWIRA HATYYGVNDS PASLGGACGY DNPYHAGFGA
70 80 90 100 110 120
HTAALSGELF RSGESCGGCY QVRCDFPADP KWCLRGAAVT VTATNFCPTN NNNGWCNLPR
130 140 150 160 170 180
HHFDMSSPAF FRIARRGNEG IVPVFYRRVG CKRRGGVRFT MRGQGNFNMV MISNVGGGGS
190 200 210 220 230 240
VRSVAVRGSK GKTWLQMTRN WGANWQSSGD LRGQRLSFKV TLTDSKTQTF LNVVPSSWWF
250
GQTFSSRGRQ FV
References and
documentation are available.
Number of amino acids: 252
Molecular weight: 27617.36
Theoretical pI: 10.06
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1214
Hydrogen H 1858
Nitrogen N 364
Oxygen O 345
Sulfur S 17
Formula: C
1214H
1858N
364O
345S
17
Total number of atoms: 3798
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54930
Abs 0.1% (=1 g/l) 1.989, assuming all pairs of Cys residues form cystines
Ext. coefficient 54430
Abs 0.1% (=1 g/l) 1.971, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.76
This classifies the protein as unstable.
Aliphatic index: 59.92
Grand average of hydropathicity (GRAVY): -0.243