ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDMTSCVARR TRSRTESYLN SILNKSKGIS GEEEDQSLGC VNSRTEKRRV NMRDACSPSP 

        70         80         90        100        110        120 
RKKKRRRRKD DDDDVVFVRT EYPEGKRDDE NVGSTSGNLQ SKSFDFGDRV CDFDADDRNL 

       130        140        150        160        170        180 
GCEEKASNFN PIDDDDDVVF VGTVQRENDH VEDDDNVGSA SVISPRVCDF DEDDAKVSGK 

       190        200        210        220        230        240 
ENPLSPDDDD DVVFLGTIAG ENQHVEDVNA GSEVCDILLD DANLRGEEKT YVSDEVVSLS 

       250        260        270        280        290        300 
SSSDDEEDPL EELGTDSREE VSGEDRDSGE SDMDEDANDS DSSDYVGESS DSSDVESSDS 

       310        320        330        340        350        360 
DFVCSEDEEG GTRDDATCEK NPSEKVYHHK KSRTFRRKHN FDVINLLAKS MLESKDVFKE 

       370        380        390        400        410        420 
DIFSWDKIAE VDSREDPVVR ESSSEKVNEH GKPRERRSFH RVREKNHLNG ESFYGGEKLC 

       430        440        450        460        470        480 
DGEETINYST EDSPPLNLRF GCEEPVLIEK TEEEKELDSL WEDMNVALTL EGMHSSTPDK 

       490        500        510        520        530        540 
NGDMLCSKGT HDFVLDDEIG LKCVHCAYVA VEIKDISPAM DKYRPSVNDN KKCSDRKGDP 

       550        560        570        580        590        600 
LPNRLEFDAS DPSSFVAPLD NIEGTVWQYV PGIKDTLYPH QQEGFEFIWK NLAGTTKINE 

       610        620        630        640        650        660 
LNSVGVKGSG GCIISHKAGT GKTRLTVVFL QSYLKRFPNS HPMVIAPATL MRTWEDEVRK 

       670        680        690        700        710        720 
WNVNIPFYNM NSLQLSGYED AEAVSRLEGN RHHNSIRMVK LVSWWKQKSI LGISYPLYEK 

       730        740        750        760        770        780 
LAANKNTEGM QVFRRMLVEL PGLLVLDEGH TPRNQSSLIW KVLTEVRTEK RIFLSGTLFQ 

       790        800        810        820        830        840 
NNFKELSNVL CLARPADKDT ISSRIHELSK CSQEGEHGRV NEENRIVDLK AMIAHFVHVH 

       850        860        870        880        890        900 
EGTILQESLP GLRDCVVVLN PPFQQKKILD RIDTSQNTFE FEHKLSAVSV HPSLYLCCNP 

       910        920        930        940        950        960 
TKKEDLVIGP ATLGTLKRLR LKYEEGVKTK FLIDFIRISG TVKEKVLVYS QYIDTLKLIM 

       970        980        990       1000       1010       1020 
EQLIAECDWT EGEQILLMHG KVEQRDRQHM IDNFNKPDSG SKVLLASTKA CSEGISLVGA 

      1030       1040       1050       1060       1070       1080 
SRVVILDVVW NPSVESQAIS RAFRIGQKRA VFIYHLMVKD TSEWNKYCKQ SEKHRISELV 

      1090       1100       1110       1120       1130 
FSSTNEKDKP INNEVVSKDR ILDEMVRHEK LKHIFEKILY HPKKSDMNTS FF 

References and documentation are available.

Number of amino acids: 1132

Molecular weight: 128343.29

Theoretical pI: 5.04

Amino acid composition: 
Ala (A)  40	  3.5%
Arg (R)  67	  5.9%
Asn (N)  59	  5.2%
Asp (D) 104	  9.2%
Cys (C)  24	  2.1%
Gln (Q)  26	  2.3%
Glu (E) 106	  9.4%
Gly (G)  63	  5.6%
His (H)  31	  2.7%
Ile (I)  53	  4.7%
Leu (L)  88	  7.8%
Lys (K)  84	  7.4%
Met (M)  22	  1.9%
Phe (F)  41	  3.6%
Pro (P)  41	  3.6%
Ser (S) 109	  9.6%
Thr (T)  48	  4.2%
Trp (W)  12	  1.1%
Tyr (Y)  23	  2.0%
Val (V)  91	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 210
Total number of positively charged residues (Arg + Lys): 151

Atomic composition:

Carbon      C	      5565
Hydrogen    H	      8796
Nitrogen    N	      1576
Oxygen      O	      1818
Sulfur      S	        46

Formula: C5565H8796N1576O1818S46
Total number of atoms: 17801

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   101770
Abs 0.1% (=1 g/l)   0.793, assuming all pairs of Cys residues form cystines


Ext. coefficient   100270
Abs 0.1% (=1 g/l)   0.781, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.80
This classifies the protein as unstable.



Aliphatic index: 75.42

Grand average of hydropathicity (GRAVY): -0.680