ProtParam
User-provided sequence:
10 20 30 40 50 60
MDNGTDSSTS KFVPEYRRTN FKNKGRFSAD ELRRRRDTQQ VELRKAKRDE ALAKRRNFIP
70 80 90 100 110 120
PTDGADSDEE DESSVSADQQ FYSQLQQELP QMTQQLNSDD MQEQLSATVK FRQILSREHR
130 140 150 160 170 180
PPIDVVIQAG VVPRLVEFMR ENQPEMLQLE AAWALTNIAS GTSAQTKVVV DADAVPLFIQ
190 200 210 220 230 240
LLYTGSVEVK EQAIWALGNV AGDSTDYRDY VLQCNAMEPI LGLFNSNKPS LIRTATWTLS
250 260 270 280 290 300
NLCRGKKPQP DWSVVSQALP TLAKLIYSMD TETLVDACWA ISYLSDGPQE AIQAVIDVRI
310 320 330 340 350 360
PKRLVELLSH ESTLVQTPAL RAVGNIVTGN DLQTQVVINA GVLPALRLLL SSPKENIKKE
370 380 390 400 410 420
ACWTISNITA GNTEQIQAVI DANLIPPLVK LLEVAEYKTK KEACWAISNA SSGGLQRPDI
430 440 450 460 470 480
IRYLVSQGCI KPLCDLLEIA DNRIIEVTLD ALENILKMGE ADKEARGLNI NENADFIEKA
490 500 510 520 530 540
GGMEKIFNCQ QNENDKIYEK AYKIIETYFG EEEDAVDETM APQNAGNTFG FGSNVNQQFN
FN
References and
documentation are available.
Number of amino acids: 542
Molecular weight: 60441.36
Theoretical pI: 4.80
Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2653
Hydrogen H 4243
Nitrogen N 733
Oxygen O 841
Sulfur S 18
Formula: C
2653H
4243N
733O
841S
18
Total number of atoms: 8488
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 56880
Abs 0.1% (=1 g/l) 0.941, assuming all pairs of Cys residues form cystines
Ext. coefficient 56380
Abs 0.1% (=1 g/l) 0.933, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.36
This classifies the protein as unstable.
Aliphatic index: 90.72
Grand average of hydropathicity (GRAVY): -0.357