ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDNILKASNM EGTSTMTVTS RSSEDSSCIS NHEQDTDTHK DGDTSGLENS KISKRKWMKE 

        70         80         90        100        110        120 
FFKLSKSPAS KSSRSIGSMK SNQSLVSMKS SDDGNSYKND YSSICGNSLP SAGLSRSNSV 

       130        140        150        160        170        180 
KELKLDSTGS QRSKNNVAML ARSSTTSQTT CSSSSSSSSY NSIKGNENDI LLQNNNHFRH 

       190        200        210        220        230        240 
NKEIPQSKGS SNINTASIMS QYNVDTQATA IMSDMQKQYD SQQMTSPFVN EDLHFDPNGE 

       250        260        270        280        290        300 
VSHVIKAIFK EIGYKYDDFS DIPVFQLMQE MYQLVKKNSS ARRTKITDYA SKLKEKEAQL 

       310        320        330        340        350        360 
KSQNDKILKL ETTNKAYKTK YKEVSLENKK IKEAFKELDN ESYNHDEELL KKYKYTRETL 

       370        380        390        400        410        420 
DRVNREQQLI IDQNEFLKKS VNELQNEVNA TNFKFSLFKE KYAKLADSIT ELNTSTKKRE 

       430        440        450        460        470        480 
ALGENLTFEC NELKEICLKY KKNIENISNT NKNLQNSFKN ERKKVLDLRN ERNLLKKEIL 

       490        500        510        520        530        540 
LIECHGSYSL LLVSNILTCY RFLLPSDTII ETESLIKELL NMNNSLSNHV SSSDEPPAEY 

       550        560        570        580        590        600 
SKRLELKCVE FEEKLLYFYQ ELVTKKIIDV IYKCFINYYK KSRQTDQKSN QNSSTPYKQS 


QRQVPHSIK 

References and documentation are available.

Number of amino acids: 609

Molecular weight: 69878.49

Theoretical pI: 8.89

Amino acid composition: 
Ala (A)  19	  3.1%
Arg (R)  20	  3.3%
Asn (N)  55	  9.0%
Asp (D)  31	  5.1%
Cys (C)   9	  1.5%
Gln (Q)  29	  4.8%
Glu (E)  49	  8.0%
Gly (G)  14	  2.3%
His (H)  10	  1.6%
Ile (I)  35	  5.7%
Leu (L)  55	  9.0%
Lys (K)  72	 11.8%
Met (M)  14	  2.3%
Phe (F)  19	  3.1%
Pro (P)  11	  1.8%
Ser (S)  85	 14.0%
Thr (T)  35	  5.7%
Trp (W)   1	  0.2%
Tyr (Y)  25	  4.1%
Val (V)  21	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      3030
Hydrogen    H	      4876
Nitrogen    N	       846
Oxygen      O	       999
Sulfur      S	        23

Formula: C3030H4876N846O999S23
Total number of atoms: 9774

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43250
Abs 0.1% (=1 g/l)   0.619, assuming all pairs of Cys residues form cystines


Ext. coefficient    42750
Abs 0.1% (=1 g/l)   0.612, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.90
This classifies the protein as unstable.



Aliphatic index: 70.76

Grand average of hydropathicity (GRAVY): -0.878