ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDNKNVVVCD NGTGYVKCGF AGENFPTSVF PCVVGRPLLR YEESLMEQQV KDIVVGETCS 

        70         80         90        100        110        120 
ELRHQLDINY PVHNGIVQNW DDMEHVWDHA FYNELKINPS DCKILLTDPP LNPSKNREKM 

       130        140        150        160        170        180 
IETMFEKYNF AGVFIQIQAV LTLYAQGLLT GLVIDSGDGV THVVPVVDGY SFPHLTKRMN 

       190        200        210        220        230        240 
VAGRHITAYL VDLLSRRGYA MNKTADFETV REIKEKLCYI SYDYKRESQL GLETTILVKN 

       250        260        270        280        290        300 
YTLPDGRVIK VGTERFQAPE ALFTPELIDV EGDGMADMVF RCIQEMDIDN RMMLYQHIVL 

       310        320        330        340        350        360 
SGGSTMYPGL PSRLEKEIQD RYLDTVLKGN KDGLKKLRLR IEDPPRRKHM VYLGGAVLAG 

       370        380 
IMKDAPEFWI NREDYMEEGI NCLNKMSQA 

References and documentation are available.

Number of amino acids: 389

Molecular weight: 44356.01

Theoretical pI: 5.41

Amino acid composition: 
Ala (A)  16	  4.1%
Arg (R)  21	  5.4%
Asn (N)  20	  5.1%
Asp (D)  27	  6.9%
Cys (C)   8	  2.1%
Gln (Q)  13	  3.3%
Glu (E)  28	  7.2%
Gly (G)  29	  7.5%
His (H)   9	  2.3%
Ile (I)  23	  5.9%
Leu (L)  37	  9.5%
Lys (K)  23	  5.9%
Met (M)  17	  4.4%
Phe (F)  13	  3.3%
Pro (P)  18	  4.6%
Ser (S)  14	  3.6%
Thr (T)  19	  4.9%
Trp (W)   3	  0.8%
Tyr (Y)  18	  4.6%
Val (V)  33	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1971
Hydrogen    H	      3103
Nitrogen    N	       529
Oxygen      O	       584
Sulfur      S	        25

Formula: C1971H3103N529O584S25
Total number of atoms: 6212

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43820
Abs 0.1% (=1 g/l)   0.988, assuming all pairs of Cys residues form cystines


Ext. coefficient    43320
Abs 0.1% (=1 g/l)   0.977, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.63
This classifies the protein as unstable.



Aliphatic index: 88.87

Grand average of hydropathicity (GRAVY): -0.287