ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR GRSSSTSPQK 

        70         80         90        100        110        120 
IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP TSSTPDSLNS TTYAPRVSSD 

       130        140        150        160        170        180 
SFTVATPLSL QSTTTRTRTR NNTVSSQITA SSSLTTDVGN ATSANIWSAN AESNTSSSPL 

       190        200        210        220        230        240 
FDYPLATSYF EPLTRFKSTD NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN 

       250        260        270        280        290        300 
RQRSQSQSTT NRVYTDAPYY QQPAQNYQVQ VPPRVPKSTS ISPVILDDVD PASINWITAN 

       310        320        330        340        350        360 
QKVPLVNQIS ALLPTNTISI SNVFPLQPTQ QHQQNAVNLT STSLATLCSQ YGKVLSARTL 

       370        380        390        400        410        420 
RGLNMALVEF STVESAICAL EALQGKELSK VGAPSTVSFA RVLPMYEQPL NVNGFNNTPK 

       430        440        450        460        470        480 
QPLLQEQLNH GVLNYQLQQS LQQPELQQQP TSFNQPNLTY CNPTQNLSHL QLSSNENEPY 

       490        500        510        520        530        540 
PFPLPPPSLS DSKKDILHTI SSFKLEYDHL ELNHLLQNAL KNKGVSDTNY FGPLPEHNSK 

       550        560        570        580        590        600 
VPKRKDTFDA PKLRELRKQF DSNSLSTIEM EQLAIVMLDQ LPELSSDYLG NTVIQKLFEN 

       610        620        630        640        650        660 
SSNIIRDIML RKCNKYLTSM GVHKNGTWVC QKIIKMANTP RQINLVTSGV SDYCTPLFND 

       670        680        690        700        710        720 
QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR AVRACLEADS IITQCQLLTI 

       730        740        750        760        770        780 
TSLIIVLSPY LATDTNGTLL ITWLLDTCTL PNKNLILCDK LVNKNLVKLC CHKLGSLTVL 

       790        800        810        820        830        840 
KILNLRGGEE EALSKNKIIH AIFDGPISSD SILFQILDEG NYGPTFIYKV LTSRILDNSV 

       850        860        870        880        890        900 
RDEAITKIRQ LILNSNINLQ SRQLLEEVGL SSAGISPKQS SKNHRKQHPQ GFHSPGRARG 

       910        920        930        940        950        960 
VSVSSVRSSN SRHNSVIQMN NAGPTPALNF NPAPMSEINS YFNNQQVVYS GNQNQNQNGN 

       970        980        990       1000       1010       1020 
SNGLDELNSQ FDSFRIANGT NLSLPIVNLP NVSNNNNNYN NSGYSSQMNP LSRSVSHNNN 

      1030       1040       1050       1060       1070 
NNTNNYNNND NDNNNNNNNN NNNNNNNNNN NNNSNNSNNN NNNDTSLYRY RSYGY 

References and documentation are available.

Number of amino acids: 1075

Molecular weight: 119508.24

Theoretical pI: 8.94

Amino acid composition: 
Ala (A)  44	  4.1%
Arg (R)  45	  4.2%
Asn (N) 144	 13.4%
Asp (D)  42	  3.9%
Cys (C)  12	  1.1%
Gln (Q)  64	  6.0%
Glu (E)  34	  3.2%
Gly (G)  43	  4.0%
His (H)  19	  1.8%
Ile (I)  60	  5.6%
Leu (L) 110	 10.2%
Lys (K)  44	  4.1%
Met (M)  12	  1.1%
Phe (F)  32	  3.0%
Pro (P)  65	  6.0%
Ser (S) 127	 11.8%
Thr (T)  78	  7.3%
Trp (W)   7	  0.7%
Tyr (Y)  35	  3.3%
Val (V)  58	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 89

Atomic composition:

Carbon      C	      5204
Hydrogen    H	      8245
Nitrogen    N	      1507
Oxygen      O	      1676
Sulfur      S	        24

Formula: C5204H8245N1507O1676S24
Total number of atoms: 16656

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    91400
Abs 0.1% (=1 g/l)   0.765, assuming all pairs of Cys residues form cystines


Ext. coefficient    90650
Abs 0.1% (=1 g/l)   0.759, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.66
This classifies the protein as unstable.



Aliphatic index: 81.41

Grand average of hydropathicity (GRAVY): -0.563