ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA 

        70         80         90        100        110        120 
ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD 

       130        140        150        160        170        180 
LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPPSMLVKD EYVHDFEGQP SLPTEGHSIQ 

       190        200        210        220        230        240 
TIQHPPSNRA STETYSAPAL LAPSESNATS TTNFPNIPVA STSQPASILA GSHSEGLLQI 

       250        260        270        280        290        300 
ASGPQPGQQQ NGFTAQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH 

       310        320        330        340        350        360 
YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD 

       370        380        390        400        410        420 
RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR 

       430        440        450        460        470        480 
APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP 

       490        500        510        520        530        540 
AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL 

       550 
HTMPIADPQP LD 

References and documentation are available.

Number of amino acids: 552

Molecular weight: 60469.24

Theoretical pI: 6.50

Amino acid composition: 
Ala (A)  50	  9.1%
Arg (R)  24	  4.3%
Asn (N)  19	  3.4%
Asp (D)  26	  4.7%
Cys (C)  14	  2.5%
Gln (Q)  32	  5.8%
Glu (E)  25	  4.5%
Gly (G)  38	  6.9%
His (H)  30	  5.4%
Ile (I)  29	  5.3%
Leu (L)  42	  7.6%
Lys (K)  20	  3.6%
Met (M)   9	  1.6%
Phe (F)  13	  2.4%
Pro (P)  47	  8.5%
Ser (S)  43	  7.8%
Thr (T)  32	  5.8%
Trp (W)   8	  1.4%
Tyr (Y)  18	  3.3%
Val (V)  33	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      2672
Hydrogen    H	      4135
Nitrogen    N	       763
Oxygen      O	       799
Sulfur      S	        23

Formula: C2672H4135N763O799S23
Total number of atoms: 8392

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71695
Abs 0.1% (=1 g/l)   1.186, assuming all pairs of Cys residues form cystines


Ext. coefficient    70820
Abs 0.1% (=1 g/l)   1.171, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.53
This classifies the protein as unstable.



Aliphatic index: 76.56

Grand average of hydropathicity (GRAVY): -0.380