ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDNVKLVKNG VLRLPPGFRF HPTDEELVVQ YLKRKVCSSP LPASIIPEFD VCRADPWDLP 

        70         80         90        100        110        120 
GNLEKERYFF STREAKYPNG NRSNRATGSG YWKATGIDKR VVTSRGNQIV GLKKTLVFYK 

       130        140        150        160        170        180 
GKPPHGSRTD WIMHEYRLSS SPPSSMGPTQ NWVLCRIFLK KRAGNKNDDD DGDSRNLRHN 

       190        200        210        220        230        240 
NNNNSSDQIE IITTDQTDDK TKPIFFDFMR KERTTDLNLL PSSPSSDHAS SGVTTEIFSS 

       250 
SDEETSSCNS FR 

References and documentation are available.

Number of amino acids: 252

Molecular weight: 28569.93

Theoretical pI: 8.97

Amino acid composition: 
Ala (A)   7	  2.8%
Arg (R)  19	  7.5%
Asn (N)  18	  7.1%
Asp (D)  20	  7.9%
Cys (C)   4	  1.6%
Gln (Q)   5	  2.0%
Glu (E)  12	  4.8%
Gly (G)  15	  6.0%
His (H)   5	  2.0%
Ile (I)  11	  4.4%
Leu (L)  17	  6.7%
Lys (K)  18	  7.1%
Met (M)   4	  1.6%
Phe (F)  12	  4.8%
Pro (P)  17	  6.7%
Ser (S)  28	 11.1%
Thr (T)  17	  6.7%
Trp (W)   4	  1.6%
Tyr (Y)   6	  2.4%
Val (V)  13	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1248
Hydrogen    H	      1954
Nitrogen    N	       364
Oxygen      O	       391
Sulfur      S	         8

Formula: C1248H1954N364O391S8
Total number of atoms: 3965

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31190
Abs 0.1% (=1 g/l)   1.092, assuming all pairs of Cys residues form cystines


Ext. coefficient    30940
Abs 0.1% (=1 g/l)   1.083, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.84
This classifies the protein as unstable.



Aliphatic index: 61.07

Grand average of hydropathicity (GRAVY): -0.836