ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDNYEGSDPW NTSSNAWTKD DDHVVSTTNS EPSLNGISGE FNTLNFSTPL DTNEEDTGFL 

        70         80         90        100        110        120 
PTNDVLEESI WDDSRNPLGA TGMSQTPNIA ANETVIDKND ARDQNIEESE ADLLDWTNNV 

       130        140        150        160        170        180 
RKTYRPLDAD IIIIEEIPER EGLLFKHANY LVKHLIALPS TSPSEERTVV RRYSDFLWLR 

       190        200        210        220        230        240 
EILLKRYPFR MIPELPPKRI GSQNADQLFL KKRRIGLSRF INLVMKHPKL SNDDLVLTFL 

       250        260        270        280        290        300 
TVRTDLTSWR KQATYDTSNE FADKKISQEF MKMWKKEFAE QWNQAASCID TSMELWYRIT 

       310        320        330        340        350        360 
LLLERHEKRI MQMVHERNFF ETLVDNFSEV TPKLYPVQQN DTILDINNNL SIIKKHLETT 

       370        380        390        400        410        420 
SSICKQETEE ISGTLSPKFK IFTDILLSLR SLFERYKIMA ANNVVELQRH VELNKEKLES 

       430        440        450        460        470        480 
MKGKPDVSGA EYDRIKKIIQ KDRRSIIEQS NRAWLIRQCI LEEFTIFQET QFLITRAFQD 

       490        500        510 
WAKLNSNHAG LKLNEWEKLV TSIMDMPISR E 

References and documentation are available.

Number of amino acids: 511

Molecular weight: 59656.65

Theoretical pI: 5.35

Amino acid composition: 
Ala (A)  22	  4.3%
Arg (R)  31	  6.1%
Asn (N)  35	  6.8%
Asp (D)  34	  6.7%
Cys (C)   3	  0.6%
Gln (Q)  19	  3.7%
Glu (E)  46	  9.0%
Gly (G)  13	  2.5%
His (H)   9	  1.8%
Ile (I)  40	  7.8%
Leu (L)  52	 10.2%
Lys (K)  35	  6.8%
Met (M)  13	  2.5%
Phe (F)  22	  4.3%
Pro (P)  20	  3.9%
Ser (S)  39	  7.6%
Thr (T)  36	  7.0%
Trp (W)  12	  2.3%
Tyr (Y)  10	  2.0%
Val (V)  20	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2650
Hydrogen    H	      4179
Nitrogen    N	       723
Oxygen      O	       811
Sulfur      S	        16

Formula: C2650H4179N723O811S16
Total number of atoms: 8379

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81025
Abs 0.1% (=1 g/l)   1.358, assuming all pairs of Cys residues form cystines


Ext. coefficient    80900
Abs 0.1% (=1 g/l)   1.356, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.45
This classifies the protein as unstable.



Aliphatic index: 85.87

Grand average of hydropathicity (GRAVY): -0.580