ProtParam
User-provided sequence:
10 20 30 40 50 60
MDPAGASVAS AIGGEGEKFA VGNIYSVKVI TGDEFRGIVM AYDPIPNFGT KPRPGHLKNT
70 80 90 100 110 120
RMVNASFITG LSYLGKTEDP LDSDNFSVDL NGLRAKEALA IRQAEADAER MGVGVTAEAQ
130 140 150 160 170 180
SIFDALSKTL PVQWENSDIL VMKEVRVRSP YLSDCVFGGT DAANNRVKKV LELERRRLQL
FGT
References and
documentation are available.
Number of amino acids: 183
Molecular weight: 19839.55
Theoretical pI: 5.54
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 873
Hydrogen H 1399
Nitrogen N 245
Oxygen O 270
Sulfur S 6
Formula: C
873H
1399N
245O
270S
6
Total number of atoms: 2793
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.578, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.578, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.47
This classifies the protein as stable.
Aliphatic index: 86.34
Grand average of hydropathicity (GRAVY): -0.196