ProtParam
User-provided sequence:
10 20 30 40 50 60
MDPCECAKTG TCNCGATCKC TNCQCTTCKK SCCSCCPSGC SKCASGCVCK GNSCGSSCCQ
References and
documentation are available.
Number of amino acids: 60
Molecular weight: 5975.98
Theoretical pI: 8.24
Total number of negatively charged residues (Asp + Glu): 2
Total number of positively charged residues (Arg + Lys): 6
Atomic composition:
Carbon C 216
Hydrogen H 367
Nitrogen N 71
Oxygen O 84
Sulfur S 21
Formula: C
216H
367N
71O
84S
21
Total number of atoms: 759
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 1250
Abs 0.1% (=1 g/l) 0.209, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.56
This classifies the protein as unstable.
Aliphatic index: 9.83
Grand average of hydropathicity (GRAVY): -0.043