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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDPCECAKTG TCNCGATCKC TNCQCTTCKK SCCSCCPSGC SKCASGCVCK GNSCGSSCCQ


References and documentation are available.

Number of amino acids: 60 Molecular weight: 5975.98 Theoretical pI: 8.24
Amino acid composition: 
Ala (A) 3 5.0% Arg (R) 0 0.0% Asn (N) 3 5.0% Asp (D) 1 1.7% Cys (C) 20 33.3% Gln (Q) 2 3.3% Glu (E) 1 1.7% Gly (G) 6 10.0% His (H) 0 0.0% Ile (I) 0 0.0% Leu (L) 0 0.0% Lys (K) 6 10.0% Met (M) 1 1.7% Phe (F) 0 0.0% Pro (P) 2 3.3% Ser (S) 8 13.3% Thr (T) 6 10.0% Trp (W) 0 0.0% Tyr (Y) 0 0.0% Val (V) 1 1.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 2 Total number of positively charged residues (Arg + Lys): 6 Atomic composition: Carbon C 216 Hydrogen H 367 Nitrogen N 71 Oxygen O 84 Sulfur S 21 Formula: C216H367N71O84S21 Total number of atoms: 759 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 1250 Abs 0.1% (=1 g/l) 0.209, assuming all pairs of Cys residues form cystines Ext. coefficient 0 Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 42.56 This classifies the protein as unstable. Aliphatic index: 9.83 Grand average of hydropathicity (GRAVY): -0.043