ProtParam
User-provided sequence:
10 20 30 40 50 60
MDPEQEISNE TLETILVSST KGSNNNNKKM EEEMKKKVSR GELGGEAQNC PRCESPNTKF
70 80 90 100 110 120
CYYNNYSLSQ PRYFCKSCRR YWTKGGTLRN VPVGGGCRRN KRSSSSAFSK NNNNKSINFH
130 140 150 160 170 180
TDPLQNPLIT GMPPSSFGYD HSIDLNLAFA TLQKHHLSSQ ATTPSFGFGG DLSIYGNSTN
190 200 210 220 230 240
DVGIFGGQNG TYNNSLCYGF MSGNGNNNQN EIKMASTLGM SLEGNERKQE NVNNNNNNSE
250 260 270 280
NPSKVFWGFP WQMTGDSAGV VPEIDPGRES WNGMVSSWNN GLLNTPLV
References and
documentation are available.
Number of amino acids: 288
Molecular weight: 31782.02
Theoretical pI: 8.11
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1363
Hydrogen H 2097
Nitrogen N 401
Oxygen O 448
Sulfur S 16
Formula: C
1363H
2097N
401O
448S
16
Total number of atoms: 4325
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41285
Abs 0.1% (=1 g/l) 1.299, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 1.287, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.03
This classifies the protein as unstable.
Aliphatic index: 50.07
Grand average of hydropathicity (GRAVY): -0.849