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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDPIGINKVL DHLAPSELIK PVKSCHNKPS VLVLDDRIVD AATKDLYVNG FQEEIQYQNP

70 80 90 100 110 120
TPENLQHMFH QGIEILDSAR MINVTHLALW KPSSFKLGNP VDFALDDNYD TFWQSDGGQP

130 140 150 160 170 180
HQLDIMFSKR MDICVMAIFF SMIADESYAP SLVKVYAGHS PSDARFYKML EVRNVNGWVA

190 200 210 220 230 240
LRFLDNREDD QLLKCQFIRL LFPVNHENGK DTHLRGIRLY VPSNEPHQDT HEWAQTLPET

250
NNVFQDAILR


References and documentation are available.

Number of amino acids: 250 Molecular weight: 28776.69 Theoretical pI: 5.38
Amino acid composition: 
Ala (A) 14 5.6% Arg (R) 11 4.4% Asn (N) 16 6.4% Asp (D) 22 8.8% Cys (C) 3 1.2% Gln (Q) 13 5.2% Glu (E) 12 4.8% Gly (G) 10 4.0% His (H) 11 4.4% Ile (I) 15 6.0% Leu (L) 25 10.0% Lys (K) 12 4.8% Met (M) 8 3.2% Phe (F) 13 5.2% Pro (P) 15 6.0% Ser (S) 14 5.6% Thr (T) 8 3.2% Trp (W) 4 1.6% Tyr (Y) 7 2.8% Val (V) 17 6.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 34 Total number of positively charged residues (Arg + Lys): 23 Atomic composition: Carbon C 1290 Hydrogen H 1980 Nitrogen N 350 Oxygen O 377 Sulfur S 11 Formula: C1290H1980N350O377S11 Total number of atoms: 4008 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 32555 Abs 0.1% (=1 g/l) 1.131, assuming all pairs of Cys residues form cystines Ext. coefficient 32430 Abs 0.1% (=1 g/l) 1.127, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 42.95 This classifies the protein as unstable. Aliphatic index: 87.72 Grand average of hydropathicity (GRAVY): -0.365