ProtParam
User-provided sequence:
10 20 30 40 50 60
MDPIGINKVL DHLAPSELIK PVKSCHNKPS VLVLDDRIVD AATKDLYVNG FQEEIQYQNP
70 80 90 100 110 120
TPENLQHMFH QGIEILDSAR MINVTHLALW KPSSFKLGNP VDFALDDNYD TFWQSDGGQP
130 140 150 160 170 180
HQLDIMFSKR MDICVMAIFF SMIADESYAP SLVKVYAGHS PSDARFYKML EVRNVNGWVA
190 200 210 220 230 240
LRFLDNREDD QLLKCQFIRL LFPVNHENGK DTHLRGIRLY VPSNEPHQDT HEWAQTLPET
250
NNVFQDAILR
References and
documentation are available.
Number of amino acids: 250
Molecular weight: 28776.69
Theoretical pI: 5.38
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1290
Hydrogen H 1980
Nitrogen N 350
Oxygen O 377
Sulfur S 11
Formula: C
1290H
1980N
350O
377S
11
Total number of atoms: 4008
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32555
Abs 0.1% (=1 g/l) 1.131, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 1.127, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.95
This classifies the protein as unstable.
Aliphatic index: 87.72
Grand average of hydropathicity (GRAVY): -0.365