ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDPLTVYKNS VKQQIDSADL LVANLVNENF VLSEKLDTKA TEIKQLQKQI DSLNAQVKEL 

        70         80         90        100        110        120 
KTQTSQQAEN SEVIKDLYEY LCNVRVHKSY EDDSGLWFDI SQGTHSGGSS DDYSIMDYKL 

       130        140        150        160        170        180 
GFVKGQAQVT EVIYAPVLKQ RSTEELYSLQ SKLPEYLFET LSFPLSSLNQ FYNKIAKSLN 

       190 
KKREKKDETE 

References and documentation are available.

Number of amino acids: 190

Molecular weight: 21751.43

Theoretical pI: 5.07

Amino acid composition: 
Ala (A)   8	  4.2%
Arg (R)   3	  1.6%
Asn (N)  10	  5.3%
Asp (D)  13	  6.8%
Cys (C)   1	  0.5%
Gln (Q)  15	  7.9%
Glu (E)  16	  8.4%
Gly (G)   6	  3.2%
His (H)   2	  1.1%
Ile (I)   8	  4.2%
Leu (L)  22	 11.6%
Lys (K)  20	 10.5%
Met (M)   2	  1.1%
Phe (F)   6	  3.2%
Pro (P)   4	  2.1%
Ser (S)  20	 10.5%
Thr (T)  10	  5.3%
Trp (W)   1	  0.5%
Tyr (Y)  10	  5.3%
Val (V)  13	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       966
Hydrogen    H	      1529
Nitrogen    N	       249
Oxygen      O	       314
Sulfur      S	         3

Formula: C966H1529N249O314S3
Total number of atoms: 3061

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.938, assuming all pairs of Cys residues form cystines


Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.938, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.30
This classifies the protein as unstable.



Aliphatic index: 85.63

Grand average of hydropathicity (GRAVY): -0.636