ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDPSGVKVLE TAEDIQERRQ QVLDRYHRFK ELSTLRRQKL EDSYRFQFFQ RDAEELEKWI 

        70         80         90        100        110        120 
QEKLQVASDE NYKDPTNLQG KLQKHQAFEA EVQANSGAIV KLDETGNLMI SEGHFASETI 

       130        140        150        160        170        180 
RTRLMELHRQ WELLLEKMRE KGIKLLQAQK LVQYLRECED VMDWINDKEA IVTSEELGQD 

       190        200        210        220        230        240 
LEHVEVLQKK FEEFQTDLAA HEERVNEVNQ FAAKLIQEQH PEEELIKTKQ EEVNAAWQRL 

       250        260        270        280        290        300 
KGLALQRQGK LFGAAEVQRF NRDVDETIGW IKEKEQLMAS DDFGRDLASV QALLRKHEGL 

       310        320        330        340        350        360 
ERDLAALEDK VKALCAEADR LQQSHPLSAN QIQVKREELI TNWEQIRTLA AERHARLDDS 

       370        380        390        400        410        420 
YRLQRFLADF RDLTSWVTEM KALINADELA NDVAGAEALL DRHQEHKGEI DAHEDSFKSA 

       430        440        450        460        470        480 
DESGQALLAA GHYASDEVRE KLSILSEERA ALLELWELRR QQYEQCMDLQ LFYRDTEQVD 

       490        500        510        520        530        540 
NWMSKQEAFL LNEDLGDSLD SVEALLKKHE DFEKSLSAQE EKITALDEFA TKLIQNNHYA 

       550        560        570        580        590        600 
MEDVATRRDA LLSRRNALHE RAMHRRAQLA DSFHLQQFFR DSDELKSWVN EKMKTATDEA 

       610        620        630        640        650        660 
YKDPSNLQGK VQKHQAFEAE LSANQSRIDA LEKAGQKLID VNHYAKEEVA ARMNEVISLW 

       670        680        690        700        710        720 
KKLLEATELK GVKLREANQQ QQFNRNVEDI ELWLYEVEGH LASDDYGKDL TNVQNLQKKH 

       730        740        750        760        770        780 
ALLEADVAAH QDRIDGITIQ ARQFQDAGHF DAENIKKKQE ALVARYEALK EPMVARKQKL 

       790        800        810        820        830        840 
ADSLRLQQLF RDVEDEETWI REKEPIAAST NRGKDLIGVQ NLLKKHQALQ AEIAGHEPRI 

       850        860        870        880        890        900 
KAVTQKGNAM VEEGHFAAED VKAKLSELNQ KWEALKAKAS QRRQDLEDSL QAQQYFADAN 

       910        920        930        940        950        960 
EAESWMREKE PIVGSTDYGK DEDSAEALLK KHEALMSDLS AYGSSIQALR EQAQSCRQQV 

       970        980        990       1000       1010       1020 
APMDDETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST NKDWWKVEVN DRQGFVPAAY 

      1030       1040       1050       1060       1070       1080 
VKKLDPAQSA SRENLLEEQG SIALRQGQID NQTRITKEAG SVSLRMKQVE ELYQSLLELG 

      1090       1100       1110       1120       1130       1140 
EKRKGMLEKS CKKFMLFREA NELQQWINEK EAALTSEEVG ADLEQVEVLQ KKFDDFQKDL 

      1150       1160       1170       1180       1190       1200 
KANESRLKDI NKVAEDLESE GLMAEEVQAV QQQEVYGMMP RDEADSKTAS PWKSARLMVH 

      1210       1220       1230       1240       1250       1260 
TVATFNSIKE LNERWRSLQQ LAEERSQLLG SAHEVQRFHR DADETKEWIE EKNQALNTDN 

      1270       1280       1290       1300       1310       1320 
YGHDLASVQA LQRKHEGFER DLAALGDKVN SLGETAQRLI QSHPESAEDL KEKCTELNQA 

      1330       1340       1350       1360       1370       1380 
WTSLGKRADQ RKAKLGDSHD LQRFLSDFRD LMSWINGIRG LVSSDELAKD VTGAEALLER 

      1390       1400       1410       1420       1430       1440 
HQEHRTEIDA RAGTFQAFEQ FGQQLLAHGH YASPEIKEKL DILDQERTDL EKAWVQRRMM 

      1450       1460       1470       1480       1490       1500 
LDHCLELQLF HRDCEQAENW MAAREAFLNT EDKGDSLDSV EALIKKHEDF DKAINVQEEK 

      1510       1520       1530       1540       1550       1560 
IAALQAFADQ LIAVDHYAKG DIANRRNEVL DRWRRLKAQM IEKRSKLGES QTLQQFSRDV 

      1570       1580       1590       1600       1610       1620 
DEIEAWISEK LQTASDESYK DPTNIQSKHQ KHQAFEAELH ANADRIRGVI DMGNSLIERG 

      1630       1640       1650       1660       1670       1680 
ACAGSEDAVK ARLAALADQW QFLVQKSAEK SQKLKEANKQ QNFNTGIKDF DFWLSEVEAL 

      1690       1700       1710       1720       1730       1740 
LASEDYGKDL ASVNNLLKKH QLLEADISAH EDRLKDLNSQ ADSLMTSSAF DTSQVKEKRD 

      1750       1760       1770       1780       1790       1800 
TINGRFQKIK SMATSRRAKL SESHRLHQFF RDMDDEESWI KEKKLLVSSE DYGRDLTGVQ 

      1810       1820       1830       1840       1850       1860 
NLRKKHKRLE AELAAHEPAI QGVLDTGKKL SDDNTIGQEE IQQRLAQFVE HWKELKQLAA 

      1870       1880       1890       1900       1910       1920 
ARGQRLEESL EYQQFVANVE EEEAWINEKM TLVASEDYGD TLAAIQGLLK KHEAFETDFT 

      1930       1940       1950       1960       1970       1980 
VHKDRVNDVC TNGQDLIKKN NHHEENISSK MKGLNGKVSD LEKAAAQRKA KLDENSAFLQ 

      1990       2000       2010       2020       2030       2040 
FNWKADVVES WIGEKENSLK TDDYGRDLSS VQTLLTKQET FDAGLQAFQQ EGIANITALK 

      2050       2060       2070       2080       2090       2100 
DQLLAAKHIQ SKAIEARHAS LMKRWTQLLA NSATRKKKLL EAQSHFRKVE DLFLTFAKKA 

      2110       2120       2130       2140       2150       2160 
SAFNSWFENA EEDLTDPVRC NSLEEIKALR EAHDAFRSSL SSAQADFNQL AELDRQIKSF 

      2170       2180       2190       2200       2210       2220 
RVASNPYTWF TMEALEETWR NLQKIIKERE LELQKEQRRQ EENDKLRQEF AQHANAFHQW 

      2230       2240       2250       2260       2270       2280 
IQETRTYLLD GSCMVEESGT LESQLEATKR KHQEIRAMRS QLKKIEDLGA AMEEALILDN 

      2290       2300       2310       2320       2330       2340 
KYTEHSTVGL AQQWDQLDQL GMRMQHNLEQ QIQARNTTGV TEEALKEFSM MFKHFDKDKS 

      2350       2360       2370       2380       2390       2400 
GRLNHQEFKS CLRSLGYDLP MVEEGEPDPE FEAILDTVDP NRDGHVSLQE YMAFMISRET 

      2410       2420       2430       2440       2450       2460 
ENVKSSEEIE SAFRALSSEG KPYVTKEELY QNLTREQADY CVSHMKPYVD GKGRELPTAF 

      2470 
DYVEFTRSLF VN 

References and documentation are available.

Number of amino acids: 2472

Molecular weight: 284637.50

Theoretical pI: 5.20

Amino acid composition: 
Ala (A) 247	 10.0%
Arg (R) 148	  6.0%
Asn (N)  97	  3.9%
Asp (D) 177	  7.2%
Cys (C)  14	  0.6%
Gln (Q) 192	  7.8%
Glu (E) 284	 11.5%
Gly (G)  95	  3.8%
His (H)  71	  2.9%
Ile (I)  90	  3.6%
Leu (L) 270	 10.9%
Lys (K) 198	  8.0%
Met (M)  50	  2.0%
Phe (F)  85	  3.4%
Pro (P)  28	  1.1%
Ser (S) 148	  6.0%
Thr (T)  90	  3.6%
Trp (W)  40	  1.6%
Tyr (Y)  41	  1.7%
Val (V) 107	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 461
Total number of positively charged residues (Arg + Lys): 346

Atomic composition:

Carbon      C	     12414
Hydrogen    H	     19705
Nitrogen    N	      3585
Oxygen      O	      3963
Sulfur      S	        64

Formula: C12414H19705N3585O3963S64
Total number of atoms: 39731

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   281965
Abs 0.1% (=1 g/l)   0.991, assuming all pairs of Cys residues form cystines


Ext. coefficient   281090
Abs 0.1% (=1 g/l)   0.988, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.70
This classifies the protein as unstable.



Aliphatic index: 79.34

Grand average of hydropathicity (GRAVY): -0.789